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Adaptor synthesis for real-time components

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Adaptor Synthesis for Real-Time Components | …

The SMART cDNA Library Construction Kit is designed for the cloning of full-length cDNA into a phage TriplEx2 vector. The kit combines the SMART technology (Switching Mechanism At 5' end of RNA Template) for cDNA amplification with adaptor-free, directional cloning into the TriplEx2 vector. This kit contains two separate protocols, allowing you to choose a method based on your starting material. The first protocol employs long-distance PCR (LD PCR), for researchers limited by their starting material. As little as 50 ng of total RNA can be used as starting material (1). The second protocol provides a more straightforward protocol for researchers with abundant amounts of starting material (i.e., 1 µg or more of poly A+ RNA). SMART libraries contain a higher percentage of full-length clones than libraries constructed by conventional methods or other full-length cDNA synthesis protocols. Thus, clones isolated from SMART cDNA libraries contain sequences corresponding to the complete 5' untranslated region of the mRNA (2).

Adaptor synthesis for real time components » Original …

The In-Fusion SMARTer Library Construction protocol can be completed in fewer steps than other library construction methods due to a highly efficient cDNA synthesis and cloning process. Only three enzymes are required to complete the entire protocol, as opposed to the usual 6–8 enzymes required for other methods. The SMART(er) cDNA synthesis protocol is user friendly and straightforward with no adaptor ligation or tailing steps. Your precious RNA is subjected to the to the least possible handling, thereby minimizing the risk of degradation.

CiteSeerX — Adaptor synthesis for real-time components

Adaptor synthesis for real-time components - CORE

...her input enabled or input insensitive, an internal clock, and control and data channels - aspects which arise from a hardware specific model. The models used in the literature on interface synthesis =-=[6, 21, 27, 23, 26, 22]-=- are quite simplistic and informal and no formal criteria have been identified for protocol compatibility or interface correctness. Using synchronous protocol automata, various notions related to inte...

Abstract. Building a real-time system from reusable or COTS components introduces several problems, mainly related to compatibility, communication, and QoS issues. We propose an approach to automatically derive adaptors in order to solve black-box integration anomalies, when possible. We consider black-box components equipped with an expressive interface that specifies the interaction behavior with the expected environment, the component clock, as well as latency, duration, and controllability of the component’s actions. The principle of adaptor synthesis is to coordinate the interaction behavior of the components in order to avoid possible mismatches, such as deadlocks. Each adaptor models the correct assembly code for a set of components. Our approach is based on labeled transition systems and Petri nets, and is implemented in a tool called SynthesisRT. We illustrate it through a case study concerning a remote medical care system. 1

Adaptor Synthesis for Real-Time Components

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