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Photosynthetic Electron Transport and ATP Synthesis

Is the production of ATP in photosynthesis similar to the ..

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Photosynthesis (Light) : Advanced Look --> 4.) ATP Synthase

DCCD (abbreviation for Dicyclohexylcarbodiimide; also known as DCC, as N,N'-dicyclohexylcarbodiimide, as Bis(cyclohexyl)carbodiimide, and as 1,3-dicyclohexylcarbodiimide) is a small organic molecule thatcan covalently modify protonated carboxyl groups. When added to ATP synthase at pH above 8, DCCD almost exclusively reacts with the carboxyl group of the conserved acidic amino acid residue of subunit (that is why subunit is sometimes called "DCCD-binding protein"). that has elevated pK and can therefore be protonated at such a high pH. Modification of the carboxyl group in a single -subunit is enough to renderthe whole -ring oligomer inactive. Because DCCD covalently binds to -subunit,this inhibition is irreversible.
The carboxyl group of the conserved amino acid residue in subunit -subunit is present inall ATP synthases known so far. So DCCD is a universal inhibitor that can FO function in bacterial, mitochondrial and chloroplast enzymes. Moreover, V- and A-type proton-transporting ATPasesare also sensitive to DCCD for the same reason. Sodium-transporting ATP synthases are also effectively inhibited by DCCD.
At lower pH (1 and inactivates it. So this compound canbe considered as an inhibitor of both FO and F1. However, inhibition of FOis highly specific, well-defined, and requires much lower DCCD concentration so usually thisinhibitor is used as FO-specific.

Photosynthetic Electron Transport and ATP Synthesis - YouTube

These results confirm earlier suggestions that the C-terminal region of ε is important in ε-CF1 and ε-CFo interactions.AB - In contrast to the well-characterized spinach (Spinacea oleracea) chloroplast ATP synthase (CF1-CFo), the properties of the chloroplast ATP synthase from pea (Pisum sativum) have not been as intensively studied.

ATP synthase, photosynthesis Flashcards | Quizlet

ATP Synthase Activation and Photosynthesis

In the of , the ATP synthase complex accomplishes the phosphorylation of ADP to ATP, providing part of the energy for subsequent biosynthesis through the .

According to the , the enzyme is called "". However, the name"ATP synthase" reflects the primary function of the enzyme moreclearly and nowadays is most wide-spread.
The other name that was commonly used in the past is "H-ATPase",sometimes a more precise "FF H-ATPase". After the discovery of many othertypes of ATP-driven proton pumps these old names are less used.
The other names that were used for ATP synthase are:

F-ATPase
FF-ATPase
F-type ATPase or simply F-ATPase
H-transporting ATPase
mitochondrial ATPase
coupling factors (F, Fand CF)
chloroplast ATPase
bacterial Ca/MgATPase
ATP synthase complex
Complex V (five)

What role does ATP synthase serve in the process of photosynthesis

What is the role of ATP synthase in photosynthesis? - 881177

It was previously shown that photosynthetic electron transfer is controlled under low CO2 via regulation of the chloroplast ATPsynthase. In the current...

protein composition, molecular sequence data, Arabidopsis thaliana, electron transfer, messenger RNA, photosynthesis, nucleotide sequences, adenosinetriphosphatase, plant proteins, thylakoids, transport proteins, alleles, cytochrome ...

How is ATP used in photosynthesis? - Quora
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Atp Synthase | Adenosine Triphosphate | Adenosine …

ATP synthesis catalyzed by ATP synthase is powered bythe transmembrane electrochemical proton potential difference, composed of twocomponents: the chemical and theelectrical one. The more protons are on one side of a membrane relativetothe other, the higher is the driving force for a proton to cross themembrane. As proton is a charged particle, its movement is alsoinfluenced by electrical field: transmembrane electrical potentialdifference will drive protons from positively charged side tothe negatively charged one. A water mill is a good analogy: the difference between the water levelsbefore and after the dam provides potential energy; downhill water flowrotates thewheel; the rotation is used to perform some work (ATP synthesis in ourcase). Quantitatively is measured in Joules per mole (J mol-1) and isdefined as:
where the "" and "" indices denote the ositively and the egatively charged sides of thecoupling membrane; is Faraday constant(96 485 C mol-1); is the molar gas constant(8.314 J mol-1K-1), is the temperature in Kelvins, and is thetransmembrane electrical potential difference involts. The value of tells, how much energy is required (or is released, depending on thedirection of the transmembrane proton flow) to move 1 mol of protonsacross the membrane.
It is often more convenient to use not , but protonmotive force ():

At room temperature (25oC) the protonmotive force (inmillivolts, as well as )is:
In the absence of transmembrane pH difference equals the transmembraneelectrical potential difference and can be directly measured by severalexperimental techniques (i.e. permeate ion distribution,potential-sensitive dyes, electrochromic carotenoid bandshift, etc.).Each pH unit of the transmembrane pH gradient corresponds to 59 mVof .
For most biological membranes engaged in ATP synthesis the value lies between 120 and 200mV ( between 11.6 and19.3 kJ mol-1).
The catalytic mechanism of ATP synthasemost probably involves rotation of Gamma subunit together with subunitEpsilon and -subunitoligomer relative to the rest of the enzyme. Such rotation wasexperimentally shown for ATP hydrolysis uncoupled to protontranslocation. Moreover, recent experiments revealed, that if Gammasubunit is mechanically forced into rotation, ATP synthesis takes placeeven without proton-translocating FO-portion.
It seems most probable that such rotation takes place . However, there is nodirect experimental evidence for such rotary mechanism in the intactenzyme under physiological conditions.
The proposed mechanism is the following:
ATP synthase activity is specifically inhibited by several compounds(both organic and inorganic). Most of these inhibitors are very toxic, so great careand appropriate safety precautions are essential when working with them (it is not very surprising thatwe get unhappy when OUR ATP synthase is blocked!).Most inhibitors are specific for either proton-translocating FO-portion, or hydrophilicF1-portion, so the section below is divided accordingly. Oligomycin is the inhibitor that gave the name "FO" to the membrane-embedded portion of ATP synthase. The subscript letter "O" in FO(not zero!) comes from Oligomycin sensitivity of this hydrophobicphosphorylation Factor in mitochondria.
Oligomycin binds on theinterface of subunit and -ring oligomer and blocks the rotary proton translocation in FO. If the enzyme is well-coupled, the activity of F1is also blocked. Because of the latter phenomenon, a subunit of mitochondrial F1-portionthat connects F1 with FO was named Oligomycin-Sensitivity Conferring Protein (OSCP).This subunit is essential for good coupling between F1 and FO and makes the ATPase activity of F1 sensitive to FO inhibitor oligomycin, hence the name.
Oligomycin is specific for mitochondrial ATP synthase and in micromolar concentrationseffectively blocks proton transport through FO. This inhibitor also works in some bacterial enzymes that show highsimilarity to mitochondrial ATP synthase, e.g. enzyme from purple bacterium . But ATP synthase from chloroplasts and from most bacteria (including )has low sensitivity to oligomycin.
It should also be noted that oligomycin in high concentrations also affects the activity of mitochondrial F1. DCCD (abbreviation for Dicyclohexylcarbodiimide; also known as DCC, as N,N'-dicyclohexylcarbodiimide, as Bis(cyclohexyl)carbodiimide, and as 1,3-dicyclohexylcarbodiimide) is a small organic molecule thatcan covalently modify protonated carboxyl groups. When added to ATP synthase at pH above 8, DCCD almost exclusively reacts with the carboxyl group of the conserved acidic amino acid residue of subunit (that is why subunit is sometimes called "DCCD-binding protein"). that has elevated pK and can therefore be protonated at such a high pH. Modification of the carboxyl group in a single -subunit is enough to renderthe whole -ring oligomer inactive. Because DCCD covalently binds to -subunit,this inhibition is irreversible.
The carboxyl group of the conserved amino acid residue in subunit -subunit is present inall ATP synthases known so far. So DCCD is a universal inhibitor that can FO function in bacterial, mitochondrial and chloroplast enzymes. Moreover, V- and A-type proton-transporting ATPasesare also sensitive to DCCD for the same reason. Sodium-transporting ATP synthases are also effectively inhibited by DCCD.
At lower pH (1 and inactivates it. So this compound canbe considered as an inhibitor of both FO and F1. However, inhibition of FOis highly specific, well-defined, and requires much lower DCCD concentration so usually thisinhibitor is used as FO-specific.

ATP synthase - Biology-Online Dictionary

The conclusion from the example above is:
The energy provided by ATP hydrolysis is not fixed (as well as theenergy necessary to synthesize ATP). Infirst approximation it depends on the concentrations of ADP, ATP, Piand on the pH. This energy increases logarithmically upon decrease inADP and Pi concentration and upon increase in ATP or H+ concentration (= decreases linearly with increase in pH). The graphs below illustrate this point, showingchange in the upon the change in theconcentrationof one reactant ( axis),assuming that the concentrations of other reactants are kept constantat values used in the example above (red dots indicate the calculated in this example).



To close up this section, I would like to note that although thethermodynamics of the ATP synthesis described here might seem rathercomplex, it is actually much more complex. One point neglected here wasthe different ADP and ATP protonation states (), the other is that the actual substrates in the reactioncatalyzed by ATP synthase are not pure nucleotides, but their magnesiumcomplexes. However, as the magnesium concentration in the living cellis relatively high and the pH is usually above 7.2, so the descriptiongiven is still applicable for thermodynamic estimates.

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