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the SMART PCR cDNA Synthesis Kit and ..

cDNA was synthesized from total RNA using the BD Biosciences SMART PCR cDNA synthesis kit.

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(Clontech Catalog #: 634902) – Super SMART PCR cDNA synthesis kit ..

The SMART cDNA Library Construction Kit is designed for the cloning of full-length cDNA into a phage TriplEx2 vector. The kit combines the SMART technology (Switching Mechanism At 5' end of RNA Template) for cDNA amplification with adaptor-free, directional cloning into the TriplEx2 vector. This kit contains two separate protocols, allowing you to choose a method based on your starting material. The first protocol employs long-distance PCR (LD PCR), for researchers limited by their starting material. As little as 50 ng of total RNA can be used as starting material (1). The second protocol provides a more straightforward protocol for researchers with abundant amounts of starting material (i.e., 1 µg or more of poly A+ RNA). SMART libraries contain a higher percentage of full-length clones than libraries constructed by conventional methods or other full-length cDNA synthesis protocols. Thus, clones isolated from SMART cDNA libraries contain sequences corresponding to the complete 5' untranslated region of the mRNA (2).

performed using the Super SMART™ PCR cDNA Synthesis Kit ..

The mRNA samples were reverse-transcribed and the resulting cDNA were amplified by a) exponential PCR amplification by Super SMART™ (BD Biosciences Clontech, Palo Alto, CA, USA), or b) linear T7 amplification through Message Amp™ aRNA kit (Ambion, Austin, TX, USA).

SMART PCR cDNA Synthesis Kit ..

Super Smart PCR cDNA Synthesis Kit ..

Total RNAs isolated from different tissues using Trizol reagent (Life Technologies Inc., Invitrogen, Carlsbad, CA, USA) were assessed by absorbance at 260 nm and 280 nm and agarose gel electrophoresis (1.5%), respectively. For construction of those full-length enriched cDNA libraries, SMART™ PCR cDNA Synthesis Kit (BD Biosciences Clontech, San Jose, CA, USA) was used for the synthesis of cDNA starting from 0.5 to 1 μg of poly (A+) RNA according to the manufacturer’s instruction. The primers were the BD Biosciences oligonucleotides SMART™ Oligo VI and CDS-3M containing the SfiI A and SfiI B recognition sequence, respectively ().

using the SMART PCR cDNA synthesis Kit ..

while 5' RACE cDNAs was produced using SMART cDNA synthesis kit (BD ..

cDNA synthesis was performed using the SMART® PCR cDNA synthesis kit ..

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    , cDNA was synthesized by using a PCR cDNA synthesis kit (SMART; Clontech, Palo Alto, CA ..

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