KW - Linoleic acid biosynthesis
sylvestris), called oleaster, which is considered an ancestor of cultivated olive trees.
T2 - Cellular and Molecular Life Sciences
Krestov Institute of Solution Chemistry of the Russian Academy of Sciences, Ivanovo, Russia, and others)
Chapter 4 - Microbial Transformations of Oleic Acid
To evaluate the essentiality of oleic acid during intraerythrocytic growth, we tested selective Δ9-desaturase inhibitors. Sterculic acid is a cyclopropene-containing FA, naturally found in plants such as Sterculia foetida, which has been shown to specifically inhibit Δ9-desaturation of stearic to oleic acid , . Recent work has revealed potent antimycobacterial activity of sterculic acid targeting the stearoyl-CoA desaturase DesA3 in Mycobacterium tuberculosis. Herein, we examined whether its methyl ester (methyl sterculate, MeSter) might inhibit oleic acid biosynthesis by targeting the P. falciparum stearoyl-CoA desaturase. In the first set of experiments, schizonts were treated with increasing concentrations of MeSter and labeled with [14C]-stearic acid. FAMEs were then extracted and developed by silver-nitrate TLC. As expected, IRBCs produced large amounts of radiolabeled oleic acid in the absence of MeSter (). This signal was strongly reduced when schizonts were treated with 100 µM MeSter, and was virtually abrogated in the presence of 1 mM MeSter. No oleic acid production was observed in uninfected RBC. These results suggest that MeSter inhibits oleic acid synthesis in vivo.
JF - Cellular and Molecular Life Sciences
Religious music accompanies these scenes and other important moments, such as when Michaela finds the article on oleic acid in a Polish scientific journal and in the scene after the partial success of the Oil and the Odones, just before the testimonials when the camera pans to a ceiling of a church adorned with frescoes of angels.
Pittaway JK, Ahuja KD, Robertson IK andBall MJ: Effects of a controlled diet supplemented with chickpeason serum lipids, glucose tolerance, satiety and bowel function. JAm Coll Nutr. 26:334–340. 2007. : :
The trans isomer of oleic acid is called elaidic acid.
These anti-inflammatory effects corroborate the observation of a lower incidence of arthritis in coastal Maoris, eating these mussels, compared with its prevalence in European or inland Maori people.
A rapid method for the preparation of C18 furanoid fatty aster from methyl ricinoleate involving dry-column chromatography was described (
Here, we have reported and characterized the activity of P. falciparum stearoyl-CoA Δ9-desaturase (PfSCD), which catalyzes the conversion of stearoyl-CoA to oleoyl-CoA. Our genome mining studies identified PFE0555w as a putative SCD. This protein showed an unusually high number of predicted transmembrane domains when compared to most mammalian SCDs. We posit that this was, at least in part, responsible for the failure to express this protein in E. coli. Several lines of evidence nevertheless indicate that this protein represents the appropriate candidate. First, PFE0555w exhibits strong similarity with other mammalian SCDs, although it possesses additional N-terminal and C-terminal extensions. Examination of the primary sequence reveals the presence of the three His boxes in regions Ia, Ib and II, a signature feature of stearoyl-CoA desaturases . Second, transgenic parasites overexpressing a PFE0555w-GFP fusion localized this protein to the ER, a feature also shared with mice, rat and yeast SCDs , . It is noteworthy that PFE0555w was originally predicted to be an apicoplast protein , however we find that the sequence lacks the typical apicoplast targeting signals. Third, we have cloned and overexpressed the central domain of PFE0555w (residues 262 to 647, termed PFE0555w-C) in M. bovis BCG, an organism whose stearoyl-CoA desaturase (DesA3) has been reported to be a target of the antitubercular thiourea drug isoxyl that inhibits oleic acid production . We observed a two-fold decrease in susceptibility to isoxyl in M. bovis BCG strains overexpressing plasmodial PFE0555w-C, when compared to the control strain (). These results are similar to those obtained by Phetsuksiri et al. , who reported that a M. bovis BCG strain overexpressing DesA3 exhibited a two-fold increase in resistance against isoxyl. This implies that the central domain of PFE0555w, like DesA3, represents a functional target of isoxyl in mycobacteria. These findings strongly suggest that PFE0555w shares a similar activity with the mycobacterial stearoyl-CoA desaturase DesA3 and lead us to posit that PFE0555w is PfSCD. Finally, functional analysis of transgenic parasites overexpressing PFE0555w clearly demonstrated a significant 2-fold increase in stearic acid to oleic acid conversion at the late ring stage, compared to the parental strain. It is noteworthy that SCDs are usually part of a multiprotein complex, along with cytochrome b5 and NADPH-cytochrome b5 reductase. Therefore, overexpression of only one partner of this complex, such as PFE0555w, may limit an important increase in SCD activity.
172.862 Oleic acid derived from tall oil fatty acids.
Lipids are molecules that do not dissolve in water, such as fats.
Korolev, Anna G.
"Lorenzo's Oil," on the whole, handles this conflict well.
Winner of Fresh Science NSW, with her research "Give chickens whole grains and feed 100 million people!".
The term "oleic" means related to, ..
FDA Regulation 21 CFR 172.862 - Oleic acid derived from tall oil fatty acids.
Biosynthesis of lipoic acid via unsaturated fatty acids.
It is recalled that In plants, they are bound to phospholipids by substituting polyunsaturated fatty acids and function as free radical scavengers suggesting their role in defense against oxidative stress.
Biosynthesis of lipoic acid via unsaturated fatty acids
We also prepared ethanol extracts of the Kabuli-typechickpeas using the same method as that used for the Desi-typechickpeas, as described in the Materials and methods, and weexamined the effects of these extracts on CPT1 and lipid contents(). The results of westernblot analysis revealed that the ethanol extracts of the Kabuli-typechicikpeas had less prominent effects on SCD1 expression, ascompared to the Deci-type ECP (). The lipid contents were reduced by the ethanolextracts of the Kabuli-type chickpeas at 0.1% (). However, this reduction wasless prominent than that induced by the Deci-type ECP ().
Lipids and their role in metabolism
Effects of ethanol extracts ofKabuli-type chickpeas on stearoyl-CoA desaturase 1 (SCD1) and lipidcontents. (A) After 8 days of culture with and without ethanolextracts of the Kabuli type or Desi type, the amount of SCD1 wasexamined by a western blot analysis (means ± SD of triplicateexperiments; **p
The dehydrogenation of stearic acid (18:0) to oleic acid ..
DeBose-Boyd RA: Feedback regulation ofcholesterol synthesis: sterolaccelerated ubiquitination anddegradation of HMG CoA reductase. Cell Res. 18:609–621. 2008. : :
with 2H-labeled oleic acid, 2H-labeled L-serine, and ..
To link the antimalarial activity of MeSter to its capacity to inhibit Δ9-desaturase-dependent oleic acid biosynthesis, we generated an isobologram to test for interactions between the inhibitor (MeSter) and the final reaction product (oleic acid) . The graph presented in (left panel) is strongly suggestive of antagonism between the two molecules . This observation is also supported by the fact that supplementation with exogenous oleic acid (25 µM) partially reverses the antimalarial activity of MeSter (, right panel). The IC50 value of oleic acid against P. falciparum was found to be around 150 µM (data not shown), consistently with a previous report . Because the fatty acid composition is extremely important to provide the appropriate physical and biological membrane properties, high concentrations of oleic acid undoubtedly alter the fatty acid composition equilibrium, ultimately leading to parasite death.
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