Call us toll-free

Indian Journal of Pharmaceutical Sciences

Polyketide - Wikipedia

Approximate price

Pages:

275 Words

$19,50

2-methyl butyric acid, 116-53-0 - The Good Scents …

Polyketides are a structurally diverse but biosynthetically related family of natural products that includes a number of medicinally important substances such as lovastatin (a cholesterol-lowering agent), erythromycin (an antibiotic), and FK506 (an immunosuppressant) []. Their structural and stereochemical complexity makes systematic chemical manipulation a formidable undertaking. Consequently, there has been considerable interest in the potential of harnessing combinatorial biosynthesis to introduce novel functionality into these bioactive compounds and to produce altogether new chemotypes.

Functional use(s) - flavor and fragrance agents. Has a acidic type odor and an fruity type flavor.

The griseorhodins belong to a family of extensively modified aromatic polyketides that exhibit activities such as inhibition of HIV reverse transcriptase and human telomerase. The vast structural diversity of this group of polyketides is largely introduced by enzymatic oxidations, which can significantly influence the bioactivity profile. Four new compounds, griseorhodins D-F, were isolated from a griseorhodin producer, Streptomyces sp. CN48+, based upon their enhancement of calcium uptake in a mouse dorsal root ganglion primary cell culture assay. Two of these compounds, griseorhodins D1 and D2, were shown to be identical to the major, previously uncharacterized products of a grhM mutant in an earlier griseorhodin biosynthesis study. Their structures enabled the establishment of a more complete hypothesis for the biosynthesis of griseorhodins and related compounds. The other two compounds, griseorhodins E and F, represent new products of post-polyketide synthase tailoring in griseorhodin biosynthesis and showed significant binding activity in a human dopamine active transporter assay.

Special Issues - Molecules - MDPI

N2 - The griseorhodins belong to a family of extensively modified aromatic polyketides that exhibit activities such as inhibition of HIV reverse transcriptase and human telomerase. The vast structural diversity of this group of polyketides is largely introduced by enzymatic oxidations, which can significantly influence the bioactivity profile. Four new compounds, griseorhodins D-F, were isolated from a griseorhodin producer, Streptomyces sp. CN48+, based upon their enhancement of calcium uptake in a mouse dorsal root ganglion primary cell culture assay. Two of these compounds, griseorhodins D1 and D2, were shown to be identical to the major, previously uncharacterized products of a grhM mutant in an earlier griseorhodin biosynthesis study. Their structures enabled the establishment of a more complete hypothesis for the biosynthesis of griseorhodins and related compounds. The other two compounds, griseorhodins E and F, represent new products of post-polyketide synthase tailoring in griseorhodin biosynthesis and showed significant binding activity in a human dopamine active transporter assay.

AB - The griseorhodins belong to a family of extensively modified aromatic polyketides that exhibit activities such as inhibition of HIV reverse transcriptase and human telomerase. The vast structural diversity of this group of polyketides is largely introduced by enzymatic oxidations, which can significantly influence the bioactivity profile. Four new compounds, griseorhodins D-F, were isolated from a griseorhodin producer, Streptomyces sp. CN48+, based upon their enhancement of calcium uptake in a mouse dorsal root ganglion primary cell culture assay. Two of these compounds, griseorhodins D1 and D2, were shown to be identical to the major, previously uncharacterized products of a grhM mutant in an earlier griseorhodin biosynthesis study. Their structures enabled the establishment of a more complete hypothesis for the biosynthesis of griseorhodins and related compounds. The other two compounds, griseorhodins E and F, represent new products of post-polyketide synthase tailoring in griseorhodin biosynthesis and showed significant binding activity in a human dopamine active transporter assay.

Molecules, an international, peer-reviewed Open Access journal.

My research lab is interested in natural product biosynthesis and biocatalysis. In the natural product area, we are interested in elucidating biosynthetic pathways of polyketides, nonribosomal peptides and related compounds. Our goal is to understand the biochemical and structural basis of different enzymes encoded in these pathways. In particular, we are studying the biosynthesis of aromatic polyketides from Streptomyces and iteratively biosynthesized compounds from filamentous fungi. By accumulating biosynthetic knowledge and enzymatic tools, we aim towards the engineered biosynthesis of unnatural natural products through combinatorial biosynthesis. In the biocatalysis area, we build upon our knowledge from the fundamental studies and aim to discover and engineer enzymes that can be used in the synthesis and semisynthesis of pharmaceutical compounds. We have demonstrated the potential of this approach in establishing a biocatalytic approach for making the blockbuster drug simvastatin. My lab has also recently become interested in research at the interface of nanotechnology, biomaterials and drug delivery. In particular, we are conducting research towards the efficient delivery of various biological molecules to cells and model animals for applications in cancer therapy, imaging, vaccination and reprogramming. These nascent efforts are performed in collaboration with various labs.

A series of 12 recombinants expressing sets of polyketide synthase (PKS) genes from the whiE (Streptomyces coelicolor), sch (S. halstedii), and cur (S. curacoi) spore pigment biosynthetic gene Clusters were prepared and shown to produce four groups of novel polyketides. Mixtures of undecaketides and dodecaketides were produced by the minimal PKS alone (TW93b, TW93c, and TW93d) or in the presence of the (unnatural) act ketoreductase (KR) (TW94b, TW94c, and TW94d), whereas when the whiE-ORFVI cyclase was present, only dodecaketides (TW95a and TW95b) arose, in high yield. This implies that the whiE minimal PKS requires an additional subunit (the cyclase) to stabilize the complex between the long nascent polyketide chain and the minimal PKS to ensure that the chain reaches the full 24 carbons. These experiments suggest that the native spore pigment is a C24 molecule with a pentacenequinone structure which is first cyclized C9 to C14. A fourth set of uncharacterized polyketides was produced when the complete set of three WhiE cyclases was expressed together with the whiE minimal PKS. It seems that the cyclases, the products of whiE-ORFs II and VII, act in concert with the remainder of the whiE PKS subunits to facilitate construction of the nearly complete spore pigment polyketide. Shortened polyketides were additionally produced by the minimal PKS alone (the heptaketide TW93a) and in the presence of the act KR (the pentaketide orcacetophenone, TW93a). While these polyketides might be derailment products resulting from a promiscuous chain length factor, they could also arise as degradation products from intermediates in the biosynthesis of the structurally related larger polyketides. Finally, the isolation of the same aromatic polyketide products from the recombinants carrying corresponding genes from the whiE, sch, and cup gene clusters suggests that the various spore pigments observed in Streptomyces spp. are derived from similar or identical polycyclic aromatic polyketide intermediates.

Order now
  • 2-methyl butyric acid, 116-53-0 - The Good Scents Company

    Biosynthesis

  • Functional use(s) - flavor and fragrance agents

    Lipid - Wikipedia

  • Has a acidic type odor and an fruity type flavor.

    KEGG PATHWAY Database - Genome

Order now

Polyketides - University of Illinois at Urbana–Champaign

The draft genome was then annotated at the RAST server, presenting an average GC content of 71.1%, 68 RNA and 7588 protein encoding genes (PEG). Within automated predicted proteins, a fraction of 31% (2320) could be assigned to 427 subsystems of the Subsystem ontology. Functions related to "Amino acids and derivatives" (16%), "Carbohydrates" (15%), "Cofactors, Vitamins, Prosthetic Groups, Pigments" (12%) and "Protein Metabolism" were the most enriched Subsystems (). Sequentially, BRA 177 annotated draft genome was mined for natural products biosynthetic gene clusters (BGCs) with the Antibiotics & Secondary Metabolite Analysis SHell (ANTISMASH). A total of 22 BGCs were detected, including pathways for production of ribosomally (lantipeptides) and non-ribosomally (NRPS) derived bioactive peptides, terpenes, siderophores and polyketides ().

polyketide compounds are common in algae, ..

Within the polyketides group, a total of 37.347 bp BGC (NODE_44) was detected, containing thirteen PEGs with homology to genes of the highly related red and mar BGCs, respectively responsible for the biosynthesis of undecylprodigiosin and its cyclic derivative streptorubin B by S. coelicolor, and mariniosins by the marine Streptomyces sp. CNQ-617 (, ).,

Order now
  • Kim

    "I have always been impressed by the quick turnaround and your thoroughness. Easily the most professional essay writing service on the web."

  • Paul

    "Your assistance and the first class service is much appreciated. My essay reads so well and without your help I'm sure I would have been marked down again on grammar and syntax."

  • Ellen

    "Thanks again for your excellent work with my assignments. No doubts you're true experts at what you do and very approachable."

  • Joyce

    "Very professional, cheap and friendly service. Thanks for writing two important essays for me, I wouldn't have written it myself because of the tight deadline."

  • Albert

    "Thanks for your cautious eye, attention to detail and overall superb service. Thanks to you, now I am confident that I can submit my term paper on time."

  • Mary

    "Thank you for the GREAT work you have done. Just wanted to tell that I'm very happy with my essay and will get back with more assignments soon."

Ready to tackle your homework?

Place an order