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Calcium oxalate formation in Lemna minor : …

KW - Calcium oxalate

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Persian - Calcium Oxalate Urolithiasis - UFAW

Calcium oxalate crystals appear to arise at focal points in most tissues. The underlying changes in oxalate metabolism and the factors associated with this crystal formation warrant further research.

Urinary saturation of calcium oxalate, brushite and undissociated uric acid did not change.

3 Methods Calcium Oxalate
Struvite
Calcium Phosphate Calcium Oxalate Mix both reactants in equimolar conditions in 200mL of DI water (0.025mol)
centrifuge for 5 minutes and wash the pellet twice centrifuging each time
Weight the pellet
Add EDTA and observe Struvite Mix all reactants in equimolar amounts in 200mL DI water (0.025mol)
Add 10mL of pH 10 buffer
centrifuge for 5 minutes and wash the pellet twice centrifuging each time
Weight the pellet
Add EDTA and observe Calcium Phosphate 4 Results Types of Stones There are five types of stones.

Persian Calcium Oxalate Urolithiasis

Calcium Oxalate
Calcium Phosphate
Uric Acid
Struvite
Cystine Reactants: Mix both reactants (0.02mol and 0.03mol)
Add 10mL of pH 10 Buffer
centrifuge for 5 minutes and wash the pellet twice centrifuging each time
Weight the pellet
Add EDTA and observe Calcium Oxalate
Struvite
Calcium Phosphate Calcium Oxalate Struvite Calcium Phosphate 5 Conclusion Kidney stones as a common health issue

Possible sources of errors

Addition of EDTA and its effect on solubility and Le Chatelier's principle.

AB - Objectives: Polymorphism of the gene for matrix GLA protein (MGP), a calcification inhibitor, is associated with nephrolithiasis. However, experimental investigations of MGP role in stone pathogenesis are limited. We determined the effect of renal epithelial exposure to oxalate (Ox), calcium oxalate (CaOx) monohydrate (COM) or hydroxyapatite (HA) crystal on the expression of MGP. Methods: MDCK cells in culture were exposed to 0.3, 0.5 or 1 mM Ox and 33, 66 or 133-150 μg/cm2 of COM/HA for 3-72 h. MGP expression and production were determined by Western blotting and densitometric analysis. Enzyme-linked immunosorbent assay was performed to determine MGP release into the medium. Hyperoxaluria was induced in male Sprague-Dawley rats by feeding hydroxyl-l-proline. Immunohistochemistry was performed to detect renal MGP expression. Results: Exposure to Ox and crystals led to time- and concentration-dependent increase in expression of MGP in MDCK cells. Cellular response was quicker to crystal exposure than to the Ox, expression being significantly higher after 3-h exposure to COM or HA crystals and more than 6 h of exposure to Ox. MGP expression was increased in kidneys of hyperoxaluric rats particularly in renal peritubular vessels. Conclusion: We demonstrate increased expression of MGP in renal tubular epithelial cells exposed to Ox or CaOx crystals as well as the HA crystals. The most significant finding of this study is the increased staining seen in renal peritubular vessels of the hyperoxaluric rats, indicating involvement of renal endothelial cells in the synthesis of MGP.

Add EDTA and observe Calcium Oxalate

Calcium oxalate kidney stones are a common condition affecting many people in the United States. The concentration of oxalate in urine is a major risk factor for stone formation. There is evidence that glyoxal metabolism may be an important contributor to urinary oxalate excretion. Endogenous sources of glyoxal include the catabolism of carbohydrates, proteins, and fats. Here, we review all the known sources of glyoxal as well as its relationship to oxalate synthesis and crystal formation.

Calcium oxalate kidney stones are a common condition affecting many people in the United States. The concentration of oxalate in urine is a major risk factor for stone formation. There is evidence that glyoxal metabolism may be an important contributor to urinary oxalate excretion. Endogenous sources of glyoxal include the catabolism of carbohydrates, proteins, and fats. Here, we review all the known sources of glyoxal as well as its relationship to oxalate synthesis and crystal formation.

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  • collect the diethyl oxalate which comes off at 160-170°C

    Excess oxalates can precipitate calcium in the bowel butare not an important factor in most diets. Figure 15.

  • dry the diethyl oxalate with anhydrous calcium chloride.

    10/01/2018 · Effect of calcium intake on urinary oxalate excretion in calcium ..

  • Oxalate induces breast cancer | BMC Cancer | Full Text

    (2011) Influence of nutrition on feline calcium oxalate urolithiasis with emphasis on endogenous oxalate synthesis.

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Oxalate Calcium Oxalate Breast Cancer ..

N2 - Objectives: Polymorphism of the gene for matrix GLA protein (MGP), a calcification inhibitor, is associated with nephrolithiasis. However, experimental investigations of MGP role in stone pathogenesis are limited. We determined the effect of renal epithelial exposure to oxalate (Ox), calcium oxalate (CaOx) monohydrate (COM) or hydroxyapatite (HA) crystal on the expression of MGP. Methods: MDCK cells in culture were exposed to 0.3, 0.5 or 1 mM Ox and 33, 66 or 133-150 μg/cm2 of COM/HA for 3-72 h. MGP expression and production were determined by Western blotting and densitometric analysis. Enzyme-linked immunosorbent assay was performed to determine MGP release into the medium. Hyperoxaluria was induced in male Sprague-Dawley rats by feeding hydroxyl-l-proline. Immunohistochemistry was performed to detect renal MGP expression. Results: Exposure to Ox and crystals led to time- and concentration-dependent increase in expression of MGP in MDCK cells. Cellular response was quicker to crystal exposure than to the Ox, expression being significantly higher after 3-h exposure to COM or HA crystals and more than 6 h of exposure to Ox. MGP expression was increased in kidneys of hyperoxaluric rats particularly in renal peritubular vessels. Conclusion: We demonstrate increased expression of MGP in renal tubular epithelial cells exposed to Ox or CaOx crystals as well as the HA crystals. The most significant finding of this study is the increased staining seen in renal peritubular vessels of the hyperoxaluric rats, indicating involvement of renal endothelial cells in the synthesis of MGP.

“Endogenous synthesis of oxalate is an important ..

Glyoxal originates from both exogenous and endogenous pathways. Exogenous sources are primarily dietary, and these include beer, wine, tea, coffee, yogurt, bread, rice, soybean paste, soy sauce, and oil []. Foods that have been fermented, roasted, baked, or fried are also a rich source of glyoxal. Environmental sources of glyoxal include cigarette smoke, smoke from residential log fires, vehicle exhaust, smog, fog, and some household cleaning products. It may also be introduced into the air during combustion of various materials. It has been found in samples of soil, groundwater, seawater, and sediment. Whether glyoxal from these sources is absorbed into the body through the gut, lungs, or skin to significantly affect glyoxal levels in tissues is not known with certainty.

7 Responses to Bone Broth, Gelatine, Oxalate, and ..

Glyoxal originates from both exogenous and endogenous pathways. Exogenous sources are primarily dietary, and these include beer, wine, tea, coffee, yogurt, bread, rice, soybean paste, soy sauce, and oil []. Foods that have been fermented, roasted, baked, or fried are also a rich source of glyoxal. Environmental sources of glyoxal include cigarette smoke, smoke from residential log fires, vehicle exhaust, smog, fog, and some household cleaning products. It may also be introduced into the air during combustion of various materials. It has been found in samples of soil, groundwater, seawater, and sediment. Whether glyoxal from these sources is absorbed into the body through the gut, lungs, or skin to significantly affect glyoxal levels in tissues is not known with certainty.

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