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Learn the story of the central dogma and how it relates to protein synthesis

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Central Dogma and Protein Synthesis - SlideShare

Peter Shen, Ph.D., and co-authors first arrived at this interesting finding when they used cryo-electron microscopy to determine the structure of the quality control proteins bound to a stalled ribosome. They observed that Rqc2p was bound to both the ribosome and a tRNA, which it had positioned adjacent to the improperly made protein chain. Seeing is not always believing, though, so the scientists involved in the study carried out numerous biochemical assays to validate this new method of protein synthesis.

A lecture presentation on the central dogma of molecular biology based on Cambell Biology.

The next steps will be to determine when this process takes place in the grand scheme of the cell cycle and what occurs when this process fails. While those key points are still unclear, what we know for sure is that our notion of how nature works is far more complicated than once perceived, and that old dogmas are subject to change with time and innovation.
The study was published in the Jan. 2, 2015, edition of Science

Central dogma of molecular biology - Wikipedia

Now, it turns out that not all the information in the DNA sequence of aorganism encodes for a protein. In fact only about 2% of the 3 billionbase pairs seem to be transcribed into RNA which can be translated into protein. The function of the rest of the DNA is at present uncertain. How does themolecular machinery of the cell know which part of the DNA encodes for proteins. It turns out that there are unique DNA sequences at the beginning and end of thepart of the DNA sequence that codes for a protein. Proceed down the DNA ofa chromosome and suddenly you come to those signals, which are recognized by thecells machinery. A complementary RNA is made from that section, and thecomplementary RNA is then decoded into a single protein. Continuefurther down the DNA sequence and another such coding sequence is found, whichcan be transcribed into a mRNA, which then can be translated into another uniqueprotein. In all there are about 30,000 such sections of DNA in allthe chromosomes that encode the information for 30,000 unique proteins. These unique coding sections of DNA that ultimately are transcribed into uniquemRNA which are translated into unique proteins are called genes. For our purposes, we conclude that one gene has the information for one protein. Each of the protein differ from each other in both length, and the specificsequence of amino acids in the protein. The DNA is indeed the blueprint of thecell. What determines the actual characteristics of the cells are the actualproteins that are made by the cell.

The binding of specific tRNAs to the ribosome is generally facilitated by the sequence of the mRNA, but this protein manages to bypass what was once perceived as a crucial step in the protein-making process. This results in a nonsensical tag of alanines and threonines added to the end of improperly made proteins.
One potential purpose of this tag may be to target the dysfunctional protein for degradation. Accumulation of degenerate proteins has been linked to neurodegenerative diseases, such as Alzheimer’s and Huntington’s. The study authors suggest this new method of protein synthesis could contribute to disease prevention.

The central dogma of molecular biology ..

Different mechanisms of termination exsist in eukayotes and prokayotes
Transcription produces RNA

rRNA -
with proteins
to form
ribosomal subunits
In Eukayotes mRNA is processed before leaving the nucleus
5'Cap and poly-A tail
Exon Splicing
A 5' cap is added to one end of the transcript.

A tail of several hundred adenines is added to the 3' end of the transcript.

These modifications function in nuclear export and maintenance of the mRNA
Eukaryotic genes contain large stretches of non-coding DNA ("
") interspersed between coding DNA ("
The introns must be removed and the exons must be spliced together prior to the movement of the mRNA transcript to the nucleus.

Alternative splicing
of exons allows eukaryotes to make multiple functional proteins from one gene
The Genetic Code:
The Ribosome
Transfer RNA - carries amino acid to the ribosome
The code is redundant and unambiguous

The code includes
The mRNA attaches to the small ribosomal subunit

Methionine is brought to the start codon AUG by the methionine tRNA

The ribosome assembles so that the start codon AUG is in the P-site to form the
initiation complex
The next codon determines the amino acid
to be brought to the ribosome.

When an error occurs, the ribosome will stall. This recruits an assembly of proteins charged with disassembling the ribosome, removing the faulty RNA and degrading the improperly made protein.
One particular quality control protein, known as Rqc2p, was found to play a surprising role. This protein — which is conserved across numerous species, from yeast to humans — binds to the stalled ribosome and promotes the continuation of protein synthesis without the use of the template mRNA sequence. Rqc2p accomplishes this unique function by binding to transfer RNA (tRNA) molecules carrying alanine or threonine amino acids.

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    What happens during translation of the central dogma in protein synthesis

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    The Central Dogma - Protein Synthesis - Prezi

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04/11/2011 · Central Dogma of Protein Synthesis

The information from the DNA, now in theform of a linear RNA sequence, is decoded in a process called translation,to form a protein, another biological polymer. The monomer in aprotein is called an amino acid, a completely different kind of molecule than anucleotide. There are twenty different naturally occurring aminoacids that differ in one of the 4 groups connected to the central carbon. In an amino acid, the central (alpha) carbon has an amine group (RNH2),a carboxylic acid group (RCO2H), and H, and an R group attached to it. Since the information in a nucleic acid (RNA) is converted into information in the form of a different molecule, a protein, this process is called translation (since the language of nucleic acids is changed to that of proteins, such as when you translate English into Chinese).

The Central Dogma of genetics is DNA to RNA to protein

The ribosome disassembles.
tRNA binding at the ribosome is mediated by complementary pairing of the anti-codon on the tRNA with the mRNA codon in the P-site
tRNA "anti-codon"
Protein Synthesis
Central process in cells
Information coded in DNA determines the sequences of amino acids in polypeptides

nontemplate strand
of the DNA will have the same sequence as the RNA transcript (except thymine replaced by uracil)
Termination in Eukayotes

The termination of transcription is different for the different polymerases.

RNA polymerase II in eukaryotes continues 1,000–2,000 nucleotides beyond the end of the gene being transcribed.

Central dogma (DNA to RNA to protein)

Anyone who has taken a biology or biochemistry class is familiar with the central dogma of the biological sciences, which describes the flow of genetic information. It dates back to Francis Crick in 1956 and can be simplified to the following concept: DNA encodes for RNA, and RNA encodes for proteins.

starts with a general overview of the central dogma of ..

In prokaryotes, the mRNA transcript is immediately translated.

In eukaryotes, the mRNA transcript is extensively processed in the nucleus before it leaves to be translated.
In Eukayotes mRNA is processed Processed before leaving the nucleus
5'Cap and poly-A tail
Exon Splicing
A modified nucleotide is added to the 5' end of the transcript.

A tail of several hundred adenine residues is put on the 3' end of the transcript.

These modifications function in nuclear export and maintenance of the mRNA
Eukaryotic genes contain large stretches of non-coding DNA ("
") interspersed between coding DNA ("
To produce a functional protein, the introns must be removed and the exons must be spliced together prior to the movement of the mRNA transcript to the nucleus.

This process is accomplished by a
(a type of enzymatic RNA molecule)
Why Introns?
It's a great question.

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