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Also use paper chromatography to ..

Cover the chromatography strip with a dark paper to protect the pigments.15.

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PIGMENT SEPARATION USING PAPER CHROMATOGRAPHY ..

Last but not least to record all the data in a table.
4.0 DATA INTERPRETATION AND DICUSSION
Band Colour
Plant Pigment
Distance moved by the pigment (mm)
Distance moved by the solvent (mm)
R
f
value
Green
ChlorophyII a
31
109
31/109=0.28
Blue Green
ChlorophyII b
80
109
80/109=0.73
Yellow
XanthophyII
86
109
86/109=0.79
Grey
Phaeophytin
92
109
92/109=0.84
Yellow
Orange
Carotene
102
109
102/109=0.94
4.1 RESULT/TABULATION OF DATA
4.0 DISCUSSION
From the experiment,the pigments that exist in the plant based on their colors were chlorophyll a which show green color, chlorophyll b which was blue green,xanthophyII which was grey and carotene which was orange in color.
the factors that affecting the movement of pigment during chromatography were porosity of the chromatography paper,solubility of the solvent as well as the molecular size of the solute.
the pigment which moved the furthest was carotene by 102 nm in distance, this indicates that the soluble pigment carotene dissolved into the acetone easiest and thus moved furthest.
it is very soluble in the solvent being used and because it forms no hydrogen bond with cellulose.
beside the large value of R
f
value also indicates that carotene was smallest in size.
The molecular size of the pigment is inversely proportional to the R
f
value.
Conclusion
Precautions
To obtain a clear and accurate chromatogram the students were advised to use gloves.

Lab 4: Photosynthesis and Chromatography of Spinach …



Drosophila, best known as vinegar fly or fruit fly, is the little fly you see flyingaround vinegar and on fruit during the fall. Why raise vinegar flies? To observe theirdevelopment, to observe the chromosomes of their salivary glands during division, toperform experiments on genetics, finally as food for amphibians that have just completedtheir metamorphosis. In this case it is necessary to breed a species that can't fly. Youcan obtain individuals with vestigial wings (wings which are not fully developed)at a university Biology or natural sciences department.
Culture medium recipe for drosophila: water 83 ml, agar-agar 0.8 g, sugar 5 g, brewer'syeast 10 g, alcohol 1.3 ml, nipagin
0.25 g. Nipagin M is used as a preservative in foods and cosmetics, like agar-agar, youcan buy it at the stores that sell science items for laboratories. Mix the yeast and thesugar, add agar-agar and water and simmer for 3 minutes. Turn off the heat. Dissolve thenipagin in alcohol and add to the rest when it has stopped smoking. Mix and let it set.
You can find other recipes at the following websites:
Aquick and simple introduction to Drosophila melanogaster
Fruit Flies - Drosophila melanogaster
Drosophila Culture (how to culture flightless fruit flies)
Observing the Development of Drosophila in Apple Juice Agar
Drosophila Genetics Lab I
A bibliography for an insect field biology course
La drosophile (in French)
Internet keywords: drosophila culture -cells, wingless fruit flies vinegar fly.

Lab 4: Photosynthesis and Chromatography of Spinach Leaves

First of all pieces of Chromatography paper was taken and aline was drawn 2cm above the bottom of the page.

How you measure the change in water level is up to you (by mass, by height). You'd get even better results if the ambient temperature was warm (eg in a fume cupboard with the heating lamps on). Research being done by Ian Craig, Erik Schmidt and Michael Scobie from the National Centre for Engineering in Agriculture (NCEA), University of Southern Queensland (USQ) into the use of these monolayers can be . I based the idea above on an article "Alternative methods for the reduction of evaporation: practical exercises for the science classroom" by Peter Schouten and colleagues from Griffith University's School of Engineering, Gold Coast, Australia. Peter has allowed me to make it available for . It was published in (2012, V47, No 2, p 202-210)


It may seem surprising but there are almost no journal articles by chemistry researchers on the effect of surface area on reaction rate - in industry or academia. Those that do relate to the area of catalysts rather than the main reactants (but that does suggest another EEI topic). The most recent paper as a stimulus for a high school chemistry EEI is one by industrial chemists Glenn Damon and Ray Cross from the Michigan College of Mining and Technology, Houghton, Michigan published in journal V28 (2) in February 1936. They reacted sulfuric acid with small squares of copper placed 2 cm under the liquid surface. However, to manipulate the surface area variable they varied the surface area of the solution exposed to the atmosphere. You could prepare a small circular piece of polystyrene foam (with a hole cut in the middle) and float it on the surface of the acid. This will give limited access of oxygen to the solution and hence limit the corrosion of the copper. It is a neat experiment and may give you a few ideas. to download it.

Hypothesis If I am to put a chromatography paper into a solvent, ..

Don't let them give you "paper clay" as it has ground up paper that stuffs things up. You'd need at least five different amounts and probably duplicates or triplicates (trials) of each. Your problem is also to control the variables: how long to stir for, how fast to stir, how long to allow settling, what to measure (height of floc, turbidity of "supernatant" liquid (clear liquid above the floc). Other variables to try: temperature, pH, salinity. Instead of clay, you could use CaCO3, BaSO4 or limewater Ca(OH)2. What might be a good Research Question? It is not much good just saying "do organic polymer flocculants clarify dirty water?" - because you know they do. Perhaps "Is PAM or PolyDADMAC better at clarifying muddy water?"

An interesting study by Drs Jenny Stauber and Mark Florence from CSIRO's, Division of Energy Chemistry, Lucas Heights Research Laboratories, Sydney, Australia found that copper ions depressed both cell division and photosynthesis in many species of algae notably the common freshwater green alga, "Chlorella" (). Reference: J. Stauber and T. Florence, 'Mechanism of toxicity of ionic copper and copper complexes to algae', 94, 511-519 (1987).

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    Chromatography Experiment Title: separation of pigments of photosynthesis using paper chromatography.

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    This experiment works very well providing care is taken over preparing the spot on the chromatography paper

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2016/11/17 · Paper Chromatography Lab ..

Students extract pigments from spinach leaves for analysis using chromatography and colorimetry. Paper chromatography separates the pigments present in the extract so they can be identified. Analysis of the extract with a colorimeter allows students to determine the relative absorbance of four different colors of light (blue, green, orange, and red). They relate the chromatography results to the colorimeter measurements to refine their understanding of how plants capture light for photosynthesis. If available, a spectrometer allows students to view the full absorbance spectrum for spinach leaves.

Documents similar to Paper Chromatography Lab Report

Today most detinning is done by an electrolytic process whereby the steel cans are soaked in a hot caustic solution and the tin electrolytically deposited onto cathode or anode plates. An earlier method has some interesting chemistry. Research done in 1930 by Scott and Davis at UCLA (California) found that tartaric acid could be used to de-tin steel cans without dissolving any of the iron. The tin could then be recovered from the stannous tartarate solution by precipitation. Their paper was published in Industrial and Engineering Chemistry, 1930, V22 (8), p 910-911. Scott and Davis found that 3 hours of immersion in a 5% tartaric acid solution with constant aeration gave optimum detinning. You could see how the concentration of tartaric acid is related to the amount of tin removed. Question: how will you aerate the solution (does an aquarium pump give you an idea)?

Biology - photosynthesis (chromatography lab report)

Students analyze spinach pigments and chloroplasts using paper chromatography, a colorimeter, and a spectrometer to understand how plants capture light for photosynthesis.

Paper chromatography lab report photosynthesis …



Industrial societies manufacture huge quantities of goods. Once used, these products arecast away. Hence, on one hand we substract great amounts of resources from nature, on theother hand we make enormous heaps of garbage which pollute the environment. If you thinkof it, a lot of materials which are discarded could be reused. In fact, metals, plasticand glass can be used in manufacturing new items. Paper and fabric can be turned back intopulp and fibers, and reassembled into new products. Organic wastes of kitchen, usuallymade of vegetable and animal substances, and garden material, can be composted and used asfertilizer. Wood can be burned, yielding electrical energy, heat and carbon dioxide whichwill be used by other plants to produce more wood. Recycling of waste materials has thedouble advantage of reducing the need for raw materials, and the amount of rubbish.
Every day for a month, separate the different kinds of scrap of your home, weight them andwrite out a list. At the end, estimate the amount of materials which can be recycled,evaluate the recycling and waste management program of your community. Highlight theproblem areas of the system. Assess the problems created by not easily recyclable things,and by the polluting ones: paint cans, batteries, oils, detergents, medicines, etc. Bringout the difficulties and the questions facing particular problems in the recovery ofwastes. Ask the authorities of your city. Write a guide for families on how treat wastesin the right manner. Show this guide and the report of your research to your teacher.
Environmental Defense, Recycling
Recycling - At School
Recycling Obscure Materials
Global Recycling Network
The Internet Consumer Recycling Guide
Plastic Bag
Recycler's World
Internet keywords: recycling.

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