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Efficient Enzymatic Synthesis of Guanosine 5′ …

THE SYNTHESIS OF GUANOSINE-5'-PHOSPHATE USING …

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Synthesis of guanosine 5′-triphosphate,3′ -diphosphate …

Phosphoenolpyruvate carboxykinase guanosine triphosphate (GTP) (EC 4.1.1.32) was synthesized by postmitochondrial supernatants of rat liver in the presence of appropriate salts, an energy supply and [ 3H]leucine. Synthesis of enzyme released from polyribosomes was detected by immunoprecipitation with specific antibody followed by electrophoresis of the dissolved antibody antigen precipitates on sodium dodecyl sulphate polyacrylamide gels in the presence of a 14C labeled enzyme marker. Enzyme synthesis in vitro occurs predominantly on free rather than bound polyribosomes. Starved animals in which deinduction of phosphoenolpyruvate carboxykinase (GTP) had been initiated by refeeding for 2 hr had a markedly decreased rate of enzyme synthesis, whether the measurements were made after injection of radioactive leucine into the intact animal or if synthesis was determined in vitro. The low rate of enzyme synthesis by liver polyribosomes from refed animals was not due to the absence of soluble factors, nor could it be increased by the addition of cyclic adenosine monophosphate to the protein synthesis system. Phosphoenolpyruvate carboxykinase (GTP) synthesis in vitro is diminished relative to total protein synthesis when the postmitochondrial supernatant is kept at 0°C for several hr before measurement of protein synthesis. Since this effect is blocked by heparin, it is probably caused by selective ribonuclease attack on enzyme messenger RNA (mRNA). Deinduction of phosphoenolpyruvate carboxykinase (GTP) is tentatively explained as being due to a transcriptional block in specific mRNA synthesis, followed by rapid degradation of existing message.

Increased Cyclic Guanosine Monophosphate Synthesis …

Cells of Escherichia coli which enter a phase of starvation for Pi induce the synthesis of the nucleotide guanosine 3',5'-bispyrophosphate (ppGpp). This induction is relA independent but depends on the spoT gene product. A mutant unable to produce ppGpp is impaired in the expression of two genes which belong to the pho regulon, a defect which is dependent on the product of spoT. We suggest that ppGpp is essential for the proper induction of the genes which belong to the pho regulon.

Synthesis of Guanosine Tetra- and Pentaphosphate …

An -acetylhexosamine 1-kinase from (NahK_15697), a guanosine 5′-diphosphate (GDP)-mannose pyrophosphorylase from (PFManC), and an inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.

AB - Phosphoenolpyruvate carboxykinase guanosine triphosphate (GTP) (EC 4.1.1.32) was synthesized by postmitochondrial supernatants of rat liver in the presence of appropriate salts, an energy supply and [ 3H]leucine. Synthesis of enzyme released from polyribosomes was detected by immunoprecipitation with specific antibody followed by electrophoresis of the dissolved antibody antigen precipitates on sodium dodecyl sulphate polyacrylamide gels in the presence of a 14C labeled enzyme marker. Enzyme synthesis in vitro occurs predominantly on free rather than bound polyribosomes. Starved animals in which deinduction of phosphoenolpyruvate carboxykinase (GTP) had been initiated by refeeding for 2 hr had a markedly decreased rate of enzyme synthesis, whether the measurements were made after injection of radioactive leucine into the intact animal or if synthesis was determined in vitro. The low rate of enzyme synthesis by liver polyribosomes from refed animals was not due to the absence of soluble factors, nor could it be increased by the addition of cyclic adenosine monophosphate to the protein synthesis system. Phosphoenolpyruvate carboxykinase (GTP) synthesis in vitro is diminished relative to total protein synthesis when the postmitochondrial supernatant is kept at 0°C for several hr before measurement of protein synthesis. Since this effect is blocked by heparin, it is probably caused by selective ribonuclease attack on enzyme messenger RNA (mRNA). Deinduction of phosphoenolpyruvate carboxykinase (GTP) is tentatively explained as being due to a transcriptional block in specific mRNA synthesis, followed by rapid degradation of existing message.

The synthesis of 2′-methylseleno adenosine and guanosine …

T1 - Increased Cyclic Guanosine Monophosphate Synthesis and Calcium Entry Blockade Account for the Relaxant Activity of the Nitric Oxide-Independent Soluble Guanylyl Cyclase Stimulator BAY 41-2272 in the Rabbit Penile Urethra

N2 - Phosphoenolpyruvate carboxykinase guanosine triphosphate (GTP) (EC 4.1.1.32) was synthesized by postmitochondrial supernatants of rat liver in the presence of appropriate salts, an energy supply and [ 3H]leucine. Synthesis of enzyme released from polyribosomes was detected by immunoprecipitation with specific antibody followed by electrophoresis of the dissolved antibody antigen precipitates on sodium dodecyl sulphate polyacrylamide gels in the presence of a 14C labeled enzyme marker. Enzyme synthesis in vitro occurs predominantly on free rather than bound polyribosomes. Starved animals in which deinduction of phosphoenolpyruvate carboxykinase (GTP) had been initiated by refeeding for 2 hr had a markedly decreased rate of enzyme synthesis, whether the measurements were made after injection of radioactive leucine into the intact animal or if synthesis was determined in vitro. The low rate of enzyme synthesis by liver polyribosomes from refed animals was not due to the absence of soluble factors, nor could it be increased by the addition of cyclic adenosine monophosphate to the protein synthesis system. Phosphoenolpyruvate carboxykinase (GTP) synthesis in vitro is diminished relative to total protein synthesis when the postmitochondrial supernatant is kept at 0°C for several hr before measurement of protein synthesis. Since this effect is blocked by heparin, it is probably caused by selective ribonuclease attack on enzyme messenger RNA (mRNA). Deinduction of phosphoenolpyruvate carboxykinase (GTP) is tentatively explained as being due to a transcriptional block in specific mRNA synthesis, followed by rapid degradation of existing message.

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  • are structurally similar to guanosine.

    Synthesis and Biochemical Characterization of N1-, N2-, and N7-Guanosine Adducts of Butadiene Monoxide Rebecca R

  • The guanine nucleoside is called guanosine

    The synthesis of 2′-methylseleno adenosine and guanosine 5′-triphosphates. In: Bioorganic & Medicinal Chemistry.

  • Journal of the Brazilian Chemical Society ..

    Guanosine - Wikipedia

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