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Long Oligos Synthesis | Biolegio

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Long Oligo Synthesis - General Biosystems

Glen Research is delighted to introduce a GalNAc modification strategy using a monomeric GalNAc support and the equivalent GalNAc phosphoramidite. Our experimental work has shown that these products are fully compatible with regular oligonucleotide synthesis and deprotection. Oligonucleotides containing GalNAc can be deprotected using standard procedures during which the acetyl protecting groups on the GalNAc group are removed. Glen Research offers these GalNAc C3 products under an agreement with AM Chemicals LLC.

You could select the following forms for Oligo synthesis and DNA Sequencing servies:

Our experiments demonstrate that a 0.05 M solution of Sulfurizing Reagent II is recommended for the synthesis of RNA phosphorothioates. A sulfurizing time of 2-4 minutes generated oligophosphorothioates of high quality. This was true for both TOM-RNA and TBDMS-RNA monomers. As shown in Figure 2, Beaucage Reagent was significantly more sluggish than Sulfurizing Reagent II. Representative HPLC analyses5 of RNA oligos are shown in Figure 3. The chromatogram on the left was obtained from sulfurizing U-TOM-RNA linkages for 60 seconds with Beaucage Reagent. The large n-1 peak is due to incomplete stepwise sulfurization and accumulation of deletions. The chromatogram on the right was an identical synthesis except using Sulfurizing Reagent II. Individual RNA sequences, especially those containing stretches of purine nucleoside residues are more difficult to sulfurize irrespective of the reagent used. To obtain a high degree of sulfurization with those oligonucleotides, a 0.1 M solution of Sulfurizing Reagent II and/or extended contact time may be required.

Long Oligo Synthesis-Gene Universal

Phosphoramidites that allow the generation of oligonucleotides containing site-specific lesions have been vital components for studying the mechanism of DNA repair. New DNA lesions are still being discovered and the study of their biological consequences will require their site-specific incorporation into oligonucleotides. The authors conclude that the increased availability of phosphoramidites for the synthesis of lesion-containing oligonucleotides should facilitate many future discoveries in the broad area of DNA damage and repair.

The authors note that the detailed studies of the molecular mechanisms of DNA repair pathways were made possible by using site-specifically modified oligonucleotides and that the availability of phosphoramidites to synthesize oligonucleotides with DNA lesions has contributed to the field. They illustrate the article using primarily structural studies in the following examples:

Gene Universal provides 81 to 125 base long oligo synthesis service

Chemical synthesis of oligodeoxyribonucleotides containing the Dewar valence isomer of the (6-4) photoproduct and their use in (6-4) photolyase studies

You will need to order your oligo through the oligo synthesis order form. Oligos ordered using our Sequencing/Oligo order form will not be given the option for normalization. Once in the oligo synthesis order form, you can select the concentration amount you would like your oligo to be normalized to.

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  • Synthesis of long oligonucleotides is demanding, ..

    Long Oligo Synthesis

  • Custom Oligo Synthesis: DNA Oligos up to 250 mer - …

    These groups are removed after synthesis of the oligonucleotide is complete, during deprotection.

  • DNA Synthesis from Oligos - OpenWetWare

    Under these conditions the coupling efficiencies are very high, thereby permitting synthesis of long oligonucleotides.

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Oligo Synthesis Services - Services - GENEWIZ

This article by Eugene Lukhtanov of ELITechGroup Molecular Diagnostics introduces the tripeptide of dihydropyrroloindole-carboxylate (CDPI3), which is a minor groove binding (MGB) moiety derived from the natural product CC-1065 and exhibits strong DNA binding properties. Synthetic oligonucleotides with covalently-attached CDPI3 moieties have enhanced DNA affinity and have improved the hybridization properties of sequence-specific DNA probes. Short CDPI3-oligonucleotides hybridize with single-stranded DNA to give more stable DNA duplexes than unmodified oligonucleotides of similar length. The author describes the general properties of CDPI3 MGB-oligonucleotide conjugates in detail, along with some specific applications:

Cambio - Excellence in Molecular Biology - Oligo Synthesis

The use of a sulfurizing reagent during the regular synthesis cycle using phosphoramidite chemistry has revolutionized the production of phosphorothioate oligonucleotide analogues. Undoubtedly, this ease of preparation of phosphorothioates has made this oligonucleotide modification by far the most common in research. Glen Research was one of the first sources of the sulfurizing reagent, 3H-1,2-benzodithiol-3-one 1,1-dioxide, popularly known as Beaucage Reagent (1).1 This sulfurizing reagent has found common use in the face of a plethora of rival reagents over the years because of its high efficiency, fast reaction time, and widespread availability. The one mild flaw we have found with Beaucage Reagent is that, although it is quite stable in acetonitrile solution in a silanized amber bottle, it is has relatively poor stability in solution once installed on the DNA synthesizer. Consequently, we have not been able to supply a sulfurizing solution, preferring to supply the powdered reagent along with an appropriate silanized bottle. The customer then weighs an appropriate amount of reagent into the silanized bottle and adds acetonitrile at a concentration of 1g/100mL. Over the years, we have considered other sulfurizing reagents but we were not able to find another reagent that exhibits the same fast sulfurization kinetics along with improved stability on the synthesizer. RNA Sulfurization

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