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" Maximizing microbial protein synthesis in the rumen "

T1 - Monensin effects on digestibility, ruminal protein escape and microbial protein synthesis on high-fiber diets.

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Ruminal Disorders and Microbial Transformations

Given the caloric benefits of additional fat and the limitations on intake during peak production, the inclusion of higher levels of fat in the diet are extremely beneficial. Much of the research is aimed at manipulation of lipid metabolism in the rumen to minimize the anti-microbial effects of fatty acids and to ameliorate disruption of ruminal fermentation.

287 Microbial protein synthesis in rumen and its importance to ruminants R.

It was concluded that inclusion of a ruminally degradable protein in the diet may synchronize release of nutrients from proteolysis with release of energy from fermentation.

Electrophoretic analysis of ruminal degradability of corn proteins.

Microbial protein synthesis and flows of nitrogen fraction to the duodenum of dairy cows.

When administered to animals these fatty acids were described to inhibit several lipoenzymes (lipoxygenase, prostaglandin synthetase) and modify tissue fatty acid composition (Liu Y et al., Lipids 1997, 32, 965).

Two acetylenic acids (6-octadecynoic and 6-nonadecynoic acids) were described in the roots of a Peruvian plant ( Rubiaceae) and were shown to inhibit the growth of fluconazole-susceptible and -resistant strains ().

Strobel, H., and J. Russell. 1986. Effect of pH and energy spilling on bacterial protein synthesis by carbohydrate-limited cultures of mixed rumen bacteria. J. Dairy Sci. 69: 2941.

Protein metabolism of ruminant animals.

Influence of carbohydrate solubility on non-protein nitrogen utilization in the ruminant.

Carbohydrate is the major component of ruminant diets and it differs widely in the rate and extent of fermentability in the rumen. In forage based diets the cell wall polysaccharides (structural carbohydrates) are the primary source of energy whereas in cereal based diets the storage polysaccharides (starch and fructosans) provide most of the energy requirements. Microbes convert both the cell wall and storage polysaccharides to five and six carbon sugars. These sugars are rapidly fermented into SCFA and can provide up to 70% of the energy requirements of the cow. Dietary ingredients have a significant impact on the proportion of acetate, propionate and butyrate produced in the rumen. Diets of dairy cattle are often supplemented with fermentable carbohydrate to meet the energy demands associated with higher milk production. Starch may be digested by either microbial or host enzymes and shifting the site of starch digestion has been the focus of intense research. The efficiency with which the three main SCFA are utilized has been suggested to be similar but theoretic estimations indicate starch digestion in the small intestine to be energetically more efficient. In contrast, performance data from several experiments show that starch fermented extensively in the rumen results in higher milk production. This suggests that increasing ruminal propionate is more beneficial in increasing the capture of fermentation energy since it reduces carbons that would be lost in methane. There may be limits to the use of starch in the lower tract and its fermentation in the rumen and maximizing starch use requires a clear understanding of those parameters. There is increasing evidence to suggest that the source of starch may result in a variable response. Feeding corn over barley has shown to alter ruminal fermentation. Barley is more rapidly fermented in the rumen and supports a higher SCFA production compared to corn. This implies that a greater proportion of carbon from barley would be made available to the cow in the form of propionate in contrast to reduced fermentation of corn in the rumen and greater capture of corn carbon as glucose in the lower tract. We know very little about how these fermentation schemes impact the energy status in the rumen and subsequent outflow of microbial protein.

Energy level, feeding frequency and pre-harvesting freezing had only minor effects on composition of harvested bacteria but differences were observed in fluid vs particle-associated or mixed populations of ruminal bacteria.
In the second study, four multiple-cannulated steers were used in a 4 $ imes$ 4 Latin square design to examine effects of forage:concentrate ratio and ruminally degradable protein supply on microbial N Kinetics and net protein synthesis.

(1981) Cloning of yeast gene for trichodermin resistance and ribosomal protein L3.
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  • Microbial Protein | What Is Microbial Protein

    Microbial protein ...

  • Microbial protein synthesis in rumen and its ..

    Relationship of Ruminal Protein and Carbohydrate Availability to Microbial Synthesis and Milk Production

  • Read "Microbial protein supply from the rumen, ..

    Regulation of protein synthesis in mammary glands of lactating dairy cows by starch and amino acids.

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Maximizing microbial protein synthesis in the rumen

The objective of this study was to determine the RDN requirement for optimum microbial protein synthesis (MPS), nitrogen capture efficiency (NCE) and nutrient utilisation in Nellore rams fed on a finger millet straw (FMS)‐based diet.

Dynamics of microbial protein synthesis in the rumen …

Microbial cells and dietary nitrogen that escapes ruminal degradation are the major sources of protein and amino acid requirements of ruminants. Although plant materials that comprise the bulk of ruminant feeds are composed of a vast array of nitrogenous compounds, most of the nitrogen contained in the forages and cereals fed to ruminants is protein. Compounds that are not true protein, but contain nitrogen are non-protein nitrogen (NPN) and include nucleic acids, nitrates and supplemental urea. Enzymatic activity in the rumen converts dietary protein into amino acids, which are in turn deaminated to ammonia and various carbon skeleton compounds (organic acids). This affects the composition of dietary protein that escapes the rumen as well as the microbial protein fraction. This process appears to be wasteful since the animal requires some amino acids for its own use. However, some plant proteins may be very indigestible by the host enzymes and these proteins often have a low content of the essential amino acids. Since the microbes synthesize protein from ammonia and other suitable carbon skeletons they contribute to the nutritional metabolism of the animal. Nucleic acids (5 to 10% of dietary N) are fermented rapidly and the nitrates are converted to nitrite, which is fermented to ammonia. The most common strategy is to increase the escape of high quality dietary protein by minimizing proteolysis, peptidolysis and deamination. It is well accepted however, that increasing the undegradable intake protein fraction must not be at the expense of lowering the degradable intake protein in the diet. Optimal protein supply to the animal depends on adequate degradable protein to maximize capture of organic matter in microbial biomass. Synchronizing the rate of nitrogen hydrolysis with the rate of energy release will increase the rate of assimilation of ammonia by microbes and maximize nitrogen use by the animal.

microbial protein synthesis, ..

Anaerobes conserve ATP in the form of a trans-membrane electrochemical gradient commonly referred to as the Proton Motive Force (PMF). However bacterial cell growth depends largely upon a membrane bound ATPase for the transfer of ATP from PMF. The rumen is a highly reduced environment and energy is often limiting. The survival of rumen microorganisms is dependent upon the efficiency with which ATP is produced, transferred and utilized during bacterial growth. Maintenance of normal fermentation within the rumen requires that the large amounts of reducing equivalents produced in the form of NADH must be re-oxidized. Microbial populations have evolved fermentation pathways that effectively lower the concentration of reducing equivalents in the rumen. The main products of these fermentation reactions are SCFA, CO2 and CH4. Acetate is the predominant SCFA found in the rumen and its formation is largely a function of the production of hydrogen from reduced cofactors. However, a high concentration of hydrogen gas is thermodynamically unfavorable and will inhibit further fermentation. Low hydrogen levels are maintained by methanogens resulting in greater hydrogen and consequently acetate production. Anaerobic conditions within the rumen result in most of the energy (ATP) in the fermented organic matter being retained in the products (SCFA and microbes) with some losses occurring in the form of CH4 and heat. The route with which reducing equivalents are disposed determines the availability of ATP. If the removal of hydrogen is coupled to acetate production, ATP yield is highest compared to ATP yields for butyrate and lactate. It is now believed that ATP yield from propionate can be comparable to that from acetate. If ATP production is uncoupled with microbial growth, excess SCFA production will be inversely related to microbial cell synthesis. This will have a major impact on the supply of the two most important sources of nutrients i.e. SCFA (energy source) and microbial biomass (protein supply).

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