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L., The Convergence of Synthetic Organic and Polymer Chemistries.

Under these conditions the coupling efficiencies are very high, thereby permitting synthesis of long oligomers.

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H., His6 tag-assisted chemical protein synthesis.

Our flexibility and expertise allow Metkinen Chemistry to cater to a range of customer needs: from one-off oligonucleotide synthesis in academia to large-scale commercial production.

Living Radical Polymerization as a Tool for the Synthesis of Polymer-Protein/Peptide Bioconjugates.

For the 0.2 µmol synthesis scale, monomer coupling is done at a 40-50-fold excess. Doing so for larger scale synthesis (such as for a 1.0 µmol scale) would be cost-prohibitive. Large-scale syntheses are done only at 10-fold mole excess of amidites. To increase the final yields of these large-scale synthesis, the coupling times are extended, so to increase coupling efficiencies.

Dr. Oligo Synthetic Chemistry Instrument - Seaview Sci

M., Synthesis of bio-inspired hybrid polymers using peptide synthesis and protein engineering.

A synthetic method for oligo(ethylene glycol) toward 44-mer (FW = 1956.35) is described. Reiteration of Williamson's ether synthesis and hydrogenation to remove protecting benzyl group affords desired oligo(ethylene glycol) toward 44-mer in moderate yields. The advantages in this method are use of commercially easily available materials as starting materials and procedures avoiding difficulty in purification of the products as much as possible.

With over 20 years of experience in oligo synthesis, Metabion can provide you with a full range of DNA oligonucleotides and modifications of highest quality at competitive prices!

Oligo synthesis: Custom DNA oligonucleotides - atdbio…

Among important areas of their application are such as design and synthesis of efficient and stable siRNAs, aptamers, anti-microRNAs (Antagomirs) as well as general use for affinity purification of RNA-binding proteins.

At present, the CATAPAZ chemistry is successfully used for the production of Universal solid support and new nucleoside bound supports for synthesis of DNA, RNA, siRNA as well as any type of modified oligonucleotides.

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  • Keck Oligo Synthesis Lab at Yale University | …

    Syntheses of Hemoprotein Models that can be Covalently Attached onto Electrode Surfaces by Click Chemistry.

  • Acetonitrile BioSyn® for DNA, RNA and Oligo Synthesis …

    J., Synthesis and Evaluation of Coumermycin A1 Analogues that Inhibit the Hsp90 Protein Folding Machinery.

  • U. Koert, Synthesis, 1995 , 115. - Organic Chemistry Portal

    H., Click chemistry as a route to cyclic tetrapeptide analogs: Synthesis of cyclo-[Pro-Val-y(triazole)-Pro-Tyr].

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Oligonucleotide synthesis - Wikipedia

Oligos are made using a DNA synthesizer, which is basically a computer-controlled reagent delivery system. The first base is attached to a solid support, usually a glass or polystyrene bead, which is designed to anchor the growing DNA chain in the reaction column. DNA synthesis consists of a series of chemical reactions.

Synthesis scales up to 1 µmol are shipped the next ..

For oligos of standard length (≤ 33 nucleotides) we guarantee a certain yield of DNA (OD260 applying to a 20-mer) to be delivered according to the respective scale of synthesis and mode of purification.

for the amount of initiating nucleotide used to begin oligo synthesis

Coupling efficiency is a way of measuring how efficiently the DNA synthesizer is adding new bases to the growing DNA chain. If every available base on the DNA chain reacted successfully with the new base, the coupling efficiency would be 100%. Few chemical reactions are 100% efficient. The industry standard for coupling efficiency during DNA synthesis is around 98,5%, with maximum coupling efficiency obtainable being around 99%. This means that at every coupling step approximately at least 1% of the available bases fail to react with the new base being added. Coupling efficiency is significantly influenced by the quality of raw material (amidites and solutions), instruments and synthesis protocols used.

Oligo Synthesis – LGC Biosearch Technologies

Coupling efficiency is important because the effects are cumulative during DNA synthesis. The Table below shows the effect of a 1% difference in coupling efficiency and how this influences the amount of full-length product, following the synthesis of oligos of different length. Considering a relatively short oligo of 20 bases, a 1% difference in coupling efficiency can result in a 15% difference, in terms of full-length final product.

Oligonucleotide synthesis, Custom Oligo Synthesis …

The table also shows that the longer an oligo, the lower the yield of full length product that can be expected, due to limitations set by chemistry. Assuming a coupling efficiency of 99% for every single base addition (industry standard is 98.5 % in average), the raw product of a 95-mer synthesis would consist of only 38.5 % full length oligonucleotide. Separating full length and failure sequences from each other by purification results in additional loss, so that low yields are a normal matter of fact.

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