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DCPIP and photosynthesis experiment question - The …

Test tube 1 and 2 both contain 2cm3 of a chloroplast suspension and 5cm3 of DCPIP.

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Photosynthesis - the Hill Reaction. Evidence for the …

An interesting EEI would be to measure the water absorption properties of the acrylate polymer using 'fake' urine (water, sodium chloride, urea, hydrochloric acid perhaps) in water in appropriate amounts. Does the nappy work equally well on individual solutions of the urine components (or are polar compounds different to non-polar ones)? How does temperature affect its properties? Where to get the polyacrylamide. You could rip open a nappy but a more controlled way would be to buy "water storage crystals" from the hardware shop. Get a Materials Safety Data Sheet (MSDS) for the one you buy to see what percentage of the crystals are polyacrylamide (should be over 90%).

Photosynthesis - the Hill Reaction

What we need is two different indicators, one to indicate the endpoint for the reaction between H+ and CO32- and the other to indicate the endpoint for the reaction between H+ and HCO3-. The first is and the second is . To calculate the amount of bicarbonate in the mixture, you subtract the amount of carbonate from the total amount of bicarbonate. The titration is quite complex because of the dissolved CO2 generated which you have to boil off. See Oliver Seeley's webpage for some great hints and cautions.

Measuring the rate of photosynthesis - Science and …

Dcpip Test

Instructions for making soap can be found easily but you'd need to work out ways (and reasons) for changing the reactants and their quantities: that is, what problem are you trying to solve, and what is your hypothesis? To keep the investigation manageable, you would be wise to consider just two independent variables (perhaps type of hydroxide and saturation of the oil) and control the rest (salt, temperature, concentrations etc). The tests might involve suds formation in hard and soft water and ability to remove an oil spot. You could add some perfume and give the leftovers to mum for Mother's Day.

It is known that for water to be electrolysed, it has to have an ionic substance added such as sodium chloride. You could see how the efficiency of the electrolysis is affected by the voltage across the electrodes, and by the concentration of salt present. You'd need to relate your results to the E° value for the non-spontaneous reaction and what happens at voltages lower than that. You may look at the changing rate of generation of the gases as time passes or at the volume after a set time. It's up to you. Does the car ad below make sense? Is it chemically feasible?

An Experiment to Investigate the Effect of Light …

26/06/2018 · The Hill Reaction depends on electrons released during the light-dependent stage of photosynthesis being picked up by the blue electron acceptor DCPIP.

In Year 8 you probably did a science experiment with tablets to see what factors affected how fast the tablets dissolved. You would have looked at temperature (tried hot and cold water), and surface area (whole tablets versus crushed up ones). However, for you would not have done it quantitatively (numerically, by calculating the surface area using a ruler). This suggests a great EEI but if you plan to do it with tablets the chances of getting an "A" will be greatly limited by how well you can control the variables. If you were to try it as an EEI you could try break up tablets into 2, 4, 6, 8 pieces and measure how long they take to dissolve and react. You could measure the sides of the chunks with a Vernier calliper and calculate the surface areas. I get a diameter of 25.63 mm, thickness of 4.30 mm and a full surface area of 1305 mm2. When split in halves I get a SA of 1461 mm2.

As the reaction proceeds in a sealed container (eg a test tube), the oxygen will be consumed and so the pressure inside the tube will decrease. If you use an oxygen pressure sensor (eg Vernier) connected to a laboratory data collector (LabQuest, Datamate, and so on) you could plot a 'percent of oxygen' versus time graph. From this you could determine the order of the reaction, or more simply, determine what factors affect the rate of reaction (temperature is an obvious one). The rate can be determined from the graph you plot.

Aim of the Experiment: To analyse the effects of light intensity on photosynthesis via the Hill reaction. Research Question: How does light intensity affec
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  • Remove several leaves from around the cut end of the stem

    04/04/2011 · Can someone explain how DCPIP and ammonium hydroxide affect the process of photosynthesis?

  • lab 4 AP sample 2 - Biology Junction

    Three test tubes were created and had water, a chloroplast solution, phosphate buffer and one of the tubes had DCPIP.

  • Pass My Exams: Revision notes for GSCE Biology, …

    Category: Papers; Title: An Experiment to Investigate the Effect of Light Intensity on the Rate of Photosynthesis

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Senior Chemistry - Extended Experimental Investigations

As photosynthesis requires light the dark conditions would prevent the DCPIP from being reduced.
Test tube 1 and two are prepared the same way as before except now both tubes are exposed to light, test tube one at 10 and test tube 2 at 25 What would you expect the observations to be?

Photosynthesis - Virtual Experiment


Test tube one would
from blue to green
as the dye in DCPIP is
decolourised when it
is reduced in
photosynthesis
Test tube two the
solution would remain blue.

Dcpip Photosynthesis - Creating My Best Life


Test tube one at 10 degrees will take longer to decolourise tha test tube two at 25 degrees due to a slower rate of photosynthesis meaning that less DCPIP is being reduced per unit time so the colour change from blue to colourless will also be quicker

and light intensity on photosynthetic ..

Your EEI could look at the fermentation rates of glucose and fructose separately - keeping everything the same except the independent variable of initial concentration. Note that 1% seems to be some sort of cut-off so examining concentrations either side of this would seem laudable. An then look at a mixture - or just look at sucrose as a natural mixture. What are you going to measure? The progress of the fermentation can be assessed by measuring the concentration of residual sugar or of the ethanol, or by the amount of CO2 produced. Sugar concentration can be measured using a Brix refractometer; or in the case of the two reducing sugars glucose and fructose, you can do a Fehling's titration; and there is a titration for ethanol. The density can also be used as an index. You could also monitor the reaction with a gas pressure sensor. I've used the Vernier sensor with a Texas Instruments CBL2 and that works well. There are lots of other gas sensors too.

light intensity on photosynthetic activity.

You would then plot acidity on the y-axis and time elapsed on the x-axis. The rate of reaction would be the slope of the line at a particular time. Does the rate vary over the whole time period? Does the rate vary as the acidity increases (is there a relationship)? Perhaps you could compare red and white wine. Does the red anthocyanin in the red wine act as an antioxidant as some people believe? What a fabulous EEI, and you'd even have some wine vinegar for your fish and chips afterwards.

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