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RP333 capsular polysaccharide biosynthesis protein CapD []

Identification and functional characterization of the putative polysaccharide biosynthesis protein (CapD) of ..

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polysaccharide biosynthesis protein capD, ..

In the present study, we identified a gene in Enterococcus faecium U0317 with homologies to the polysaccharide biosynthesis protein CapD that is made up of 336 amino acids and putatively catalyzes N-linked glycosylation.

Polysaccharide biosynthesis protein CapD

Results of these analysis revealed the highly virulent H. parasuis serovar 4 lacks genes encoding for, glycosyl transferases, polysaccharide biosynthesis protein capD, spore coat polysaccharide biosynthesis protein C, polysaccharide export protein and sialyltransferase which can modify the lipopolysaccharide forming a short-chain LPS lacking O-specific polysaccharide chains often referred to as lipooligosaccharide (LOS). In addition, it can modify the outer membrane protein (OMP) structure. The lack of sialyltransferase significantly reduced the amount of sialic acid incorporated into LOS, a major and essential component of the cell wall and an important virulence determinant. These molecules may be involved in various stages of pathogenesis through molecular mimicry and by causing host cell cytotoxicity, reduced inflammatory and immunological response to infection with this organism. The mechanism by which sialyation of LPS contributes to virulence is a key to understanding the pathogenesis of this highly virulent H. parasuis serovar 4. This analysis also revealed the presence of virulence associated genes similar to the MerR family transcriptional regulators, macrophage infectivity potentiator protein, hemolysin, opacity associated protein, toxin antitoxin system, and virulence associated protein D and colicins. Haemophilus parasuis serovar 4 variants also possess extensive metal ion uptake and regulation mechanism which controls various virulence and virulence associated genes. A combination of virulence associated factors and/or genes and proteins with overlapping functions may be responsible for the apparent enhanced virulence of this organism.

0 0 340 B7H2I4 Vi polysaccharide biosynthesis protein vipB/tviC OS ..

CMP-N-acetylneuraminate synthase, and candidates for polysaccharide biosynthesis protein CapD.

Polysaccharide export protein and polysaccharide biosynthesis protein are located as contiguous ORFs in serovar 4 and serovar 5 strains. Bacteria export extracellular polysaccharides (EPS) and capsular polysaccharides (CPS) through complex network of transport systems (). These biopolymers have various biological functions, such as providing reserve material or as part of the cell protective structure, and can provide an advantage for pathogenic bacteria under certain environmental conditions.

Results of these analysis revealed the highly virulent H. parasuis serovar 4 lacks genes encoding for, glycosyl transferases, polysaccharide biosynthesis protein capD, spore coat polysaccharide biosynthesis protein C, polysaccharide export protein and sialyltransferase which can modify the lipopolysaccharide forming a short-chain LPS lacking O-specific polysaccharide chains often referred to as lipooligosaccharide (LOS). In addition, it can modify the outer membrane protein (OMP) structure. The lack of sialyltransferase significantly reduced the amount of sialic acid incorporated into LOS, a major and essential component of the cell wall and an important virulence determinant. These molecules may be involved in various stages of pathogenesis through molecular mimicry and by causing host cell cytotoxicity, reduced inflammatory and immunological response to infection with this organism. The mechanism by which sialyation of LPS contributes to virulence is a key to understanding the pathogenesis of this highly virulent H. parasuis serovar 4. This analysis also revealed the presence of virulence associated genes similar to the MerR family transcriptional regulators, macrophage infectivity potentiator protein, hemolysin, opacity associated protein, toxin antitoxin system, and virulence associated protein D and colicins. Haemophilus parasuis serovar 4 variants also possess extensive metal ion uptake and regulation mechanism which controls various virulence and virulence associated genes. A combination of virulence associated factors and/or genes and proteins with overlapping functions may be responsible for the apparent enhanced virulence of this organism.

Pjdr2_0353 polysaccharide biosynthesis protein CapD: ..

Pjdr2_0411 polysaccharide biosynthesis protein: confidence=66 n=10: Directon coords: 495794 - …

In pathogenic bacteria, production and export of EPS and CPS poses a major challenge as these high-molecular-weight hydrophilic polymers must be assembled and exported across the envelope, without compromising the essential functional properties of the envelope (). Two major pathways with different polymer biosynthesis strategies are employed by bacteria in the assembly of most EPS/CPS: the Wzy-dependent and ATP-binding cassette (ABC) transporter-dependent pathways (). They converge in an outer membrane export step mediated by a member of the outer membrane auxiliary (OMA) protein family. Outer membrane auxiliary proteins form outer membrane efflux channels for the biopolymers. The polysaccharide co-polymerase (PCP) family of enzymes interacts with OMA proteins forming a trans-envelope scaffold for polymer export (). The variant serovar 4 strains have numerous ABC transporter systems similar to virulent serovar 5 but lack the polysaccharide export protein (HPM_0299) which reveals a functional overlap between ABC transporters in exporting complex carbohydrates and biopolymers.

The D gene (HPM_0300) encodes a polysaccharide biosynthesis protein which has been implicated in virulence. However, a characteristic feature of this gene that is associated with the pathogenicity of is not understood (). This domain is found in diverse bacterial polysaccharide biosynthetic proteins including the WalL protein, mannosyl-transferase and epimerases (). The CapD protein is required for biosynthesis of type 1 capsular polysaccharide in spp. () and serum-resistance in (). Despite lacking this domain the variant serovar 4-1 and serovar 4-2 strains are highly virulent and were recovered in pure cultures from experimentally infected dead pigs, possibly supporting serum-resistance and virulence without the capD protein.

BRADO5199: putative polysaccharide biosynthesis protein (capD-like) ..
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  • exopolysaccharide biosynthesis protein gene of pBC218 derived ..

    putative capsular polysaccharide biosynthesis protein; BRADO5153: nucleotide sugar …

  • Required for the biosynthesis of type 1 capsular polysaccharide.

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  • glycosyl transferase family protein (334 aa) ..

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Coagulase-Negative Staphylococci

This domain is found in diverse bacterial polysaccharide biosynthesis proteins including the CapD protein from Staphylococcus aureus [], the WalL protein, mannosyl-transferase [], and several putative epimerases. The CapD protein is required for biosynthesis of type 1 capsular polysaccharide.

Characterization of the Structure and Biological …

32. Wang X, Xu X, Wu Y, Li L, Cao R, Cai X, Chen H. Polysaccharide biosynthesis protein CapD is a novel pathogenicity-associated determinant of involved in serum-resistance ability. 2013;164:184-189

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