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Semisynthesis of proteins by expressed protein ligation

21/12/2017 · Expressed Protein Ligation: Method and Applications

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Protein Semisynthesis And Expressed Protein Ligation

N2 - A protein semisynthesis method - expressed protein ligation - is described that involves the chemoselective addition of a peptide to a recombinant protein. This method was used to ligate a phosphotyrosine peptide to the C terminus of the protein tyrosine kinase C-terminal Src kinase (Csk). By intercepting a thioester generated in the recombinant protein with an N- terminal cysteine containing synthetic peptide, near quantitative chemical ligation of the peptide to the protein was achieved. The semisynthetic tail- phosphorylated Csk showed evidence of an intramolecular phosphotyrosine-Src homology 2 interaction and an unexpected increase in catalytic phosphoryl transfer efficiency toward a physiologically relevant substrate compared with the non-tail-phosphorylated control. This work illustrates that expressed protein ligation is a simple and powerful new method in protein engineering to introduce sequences of unnatural amino acids, posttranslational modifications, and biophysical probes into proteins of any size.

"Protein semisynthesis and expressed protein ligation: chasing a ..

This work illustrates that expressedprotein ligation is a simple and powerful new method in proteinengineering to introduce sequences of unnatural amino acids,posttranslational modifications, and biophysical probes into proteins ofany size.

Semisynthesis Of Proteins By Expressed Protein Ligation

Expressed protein ligation for the preparation of fusion proteins with cell penetrating peptides for endotoxin removal and intracellular delivery Biochim Biophys Acta .

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- A protein semisynthesis method—expressed protein ligation ..

A protein semisynthesis method-expressed protein ligation-is describedthat involves the chemoselective addition of a peptide to a recombinantprotein.

Expressed protein ligation is a valuable method for protein semisynthesis that involves the reaction of ... Semisynthesis using expressed protein ligation ... Expressed protein ligation for protein semisynthesis and ... Expressed protein ligation for protein semisynthesis and ... of highly pure recombinant proteins. Expressed protein ligation extends the scope of NCL by ... Expressed protein ligation - David - 2004 - European Journal ... ... A. G. (2004), Expressed protein ligation. European ... C.J. (1995) Peptide ligation and semisynthesis ... of bacterially expressed proteins using modified ... Semisynthesis and segmental isotope labeling of the apoE3 N ... Semisynthesis and segmental isotope labeling of the apoE3 N-terminal domain using expressed protein ligation. ... Protein semisynthesis and expressed protein ligation ... Expressed Protein Ligation for Protein Semisynthesis and ... Key Words: Expressed protein ligation (EPL); intein; protein engineering; ... Expressed Protein Ligation for Protein Semisynthesis and Engineering Book Title Semisynthesis of Proteins by Expressed Protein Ligation ... Abstract Expressed protein ligation ... The term protein semisynthesis originally referred to processes in which proteolytic or chemical cleavage fragments ... Semisynthesis of proteins by expressed protein ligation Expressed protein ligation (EPL) is a protein engineering approach that allows recombinant and synthetic polypeptides to be chemoselectively and regioselectively ... Protein semisynthesis and expressed protein ligation ... Protein semisynthesis and expressed protein ligation >>> next page Christopher alexander notes on the synthesis Studies of ion channels using expressed protein ligation Expressed protein ligation (EPL) is a semisynthetic technique for the chemoselective ligation of a synthetic peptide to a recombinant peptide that results in a native ... Intein - Wikipedia An intein is a segment of a protein that is able to excise itself and ... Inteins have been engineered for particular applications such as protein semisynthesis ...

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  • Next-Generation DNA Sequencing Methods | Annual …

    Semisynthesis of Dimeric Proteins by Expressed Protein Ligation Barbara Ziaco,† Soccorsa Pensato,† Luca D

  • expressed protein ligation (EPL), ..

    including protein semisynthesis and site-specific labeling [3,7, Intein Mediated Protein Ligation ..

  • 19/12/2017 · Semisynthesis of pro..

    Protein - Wikipedia

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16/03/2012 · Expressed protein ligation ..

Protein semisynthesis or expressed protein ligation combine SPPS and protein expression to generate proteins endowed with any imaginable modification. We have used this technique to introduce amide-to-ester backbone modifications into proteins in order to investigate backbone hydrogen bonds and site-specific phosphorylation, and the impact of these PTMs on PPIs.

27/01/2004 · Expressed protein ligation

Key technologies the Strømgaard lab comprise solid-phase peptide synthesis (SPPS), protein expression and purification, and chemical protein ligation. In addition, we employ a wide range of state-of-the-art technologies for modern peptide and protein engineering:

Expressed protein ligation - Revolvy

Expressed Protein Ligation, ... Expressed protein ligation is a novel protein semisynthesis method that permits the in vitro ligation of a chemically synthesized ... Expressed Protein Ligation for Protein Semisynthesis and ... Read "Expressed Protein Ligation for Protein Semisynthesis and Engineering" on DeepDyve ... Expressed protein ligation (EPL); ... Expressed Enzymatic Ligation for the Semisynthesis of ... Expressed Enzymatic Ligation for the Semisynthesis of Chemically Modified Proteins ... and expressed protein ligation were combined in a ... for Semisynthesis, ... Expressed protein ligation: A general method for protein ... Expressed protein ligation: A general method for ... ABSTRACT A protein semisynthesis method—expressed protein ligation—is described that involves the chemoselective Selenocysteine in Native Chemical Ligation and Expressed ... Selenocysteine in Native Chemical Ligation and Expressed Protein Ligation ... semisynthesis. Many natural proteins contain selenocysteine residues. For SpringerProtocols: Abstract: Expressed Protein Ligation for ... Expressed Protein Ligation for Protein Semisynthesis and ... of highly pure recombinant proteins. Expressed protein ligation extends the scope ... Expressed Protein Ligation to Study Protein Interactions ... Protein & Peptide Letters ISSN (Print): 0929-8665 ISSN ... Expressed Protein Ligation to Study Protein Interactions: Semi-Synthesis of the G-Protein Alpha Subunit. Expressed protein ligation: A general method for protein ... Expressed Protein Ligation. See Fig. Fig.2 2 for the overall strategy. Primers containing an NdeI site (upstream) and a SmaI site (downstream) were used to PCR ... Semisynthesis of Ribonuclease A using Intein- Mediated ... KEY WORDS: intein-mediated protein ligation, semisynthesis, protein chimera, ribonuclease A, ... The recently established method of expressed protein ligation ... Semi-Synthesis of Labeled Proteins for Spectroscopic Applications ligation in the 90s and then of expressed protein ligation ... The great advantage of chemical protein synthesis and semi ... large proteins. 1.2. Protein Synthesis ...

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