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Protein Engineering: Design and synthesis of a protein.

Incorporation of T and B epitopes of the circumsporozoite protein in a chemically defined synthetic vaccine against malaria.

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Custom Peptide Synthesis Service; Protein Isolation ..

Fokin, Rapid Discovery and Structure-Activity Profiling of Novel Inhibitors of HIV-1 Protease Enabled by the Copper(I)-Catalyzed Synthesis of 1,2,3-Triazoles and Their Further Functionalization,

Synthesis and application of unprotected cyclic peptides as building blocks for peptide dendrimers.

Introduction – Different kinds of Fire and classifications; Fire Science – The Basic Principles; The Fundamental elements of Fire Protection Engineering; Building Materials and Design; Water Supply for Fire Protection; Fire Extinguishing Systems; Fire Alarm System; Special Occupancies and Hazards; Occupancies Hazard Classification System; Fire Flow Demand for Unsprinklered Facilities; Performance Based Fire Safety Design; Codes and Regulations

What Is Protein Synthesis - Protein Synthesis

This is an advanced course once the students are familiar with basic synthetic chemistry principles. Reactions of heterocyclic compounds (which form the basic skeleton of natural products) includes imidazoles, oxazoles, thiazoles, pyridazines, pyrimidines and pyrazines, terpenes, alkaloids, antibiotics, carbohydrates, vitamins, nucleic acids and proteins. This course will also offer biosynthetic pathway of some important natural products.

Biosynthesis:
Synthesis of biomolecules (carbohydrates, lipids and proteins) from common building blocks; transport and regulation of synthetic precursors; common intermediates in synthesis of other biomolecules; synthesis of nucleic acid components

Chemical synthesis of proteins - ScienceDirect

Fundamentals of recombinant DNA technology, Cloning vectors, Genetic transformation of prokaryotes, PCR technologies, sequencing techniques; Prokaryotic gene expression systems, fusion proteins constructs, Fungus based expression systems, Insect cell expression systems, Mammalian cell expression systems; Directed mutagenesis and protein engineering; Synthesis of commercial products such as small biological molecules, antibiotics and biopolymers by recombinant microorganisms.

Metabolic Pathways and Control:
Location of carbohydrate, lipid and protein metabolism pathways with respect to one another; common metabolic sources and fates; compartmentalization of metabolic pathways; regulation of carbohydrate, lipid and protein metabolism; synthesis, storage and utilization of energy sources; thermodynamic relationships among pathways

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  • Protein Engineering: Design and synthesis of a protein.

    This protocol describes the synthesis of peptides for affinity testing and bioconjugate with ..

  • Protein Ligation Synthesis Reagents;

    There are two main protocols that have been used for the solid-phase chemical synthesis ..

  • Peptide Synthesis —BIO-PROTOCOL

    shows the optimized synthesis of the neurosecretory protein GL composed of ..

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Solid phase, Peptide, Fmoc, Scaffold protein, Microcleavage.

Availability of and extraction of biomolecules from scene of interest. DNA collection and quantitation from samples. Protein Based Markers (from blood, serum, tissues). Amplifying DNA through Polymerase Chain Reaction (PCR), multiplex PCR, optimizing PCR, DNA sequencing, applications for DNA molecules, technologies for separation of DNA; Unique DNA sequences for identification, variable number tandem repeats (VNTRs) and short tandem repeats (STRs); Interpreting data, storage and mining in the context of DNA typing; Introduction to forensic DNA databases; statistical interpretation of biomolecular forensics; statistical genetics; Y-chromosome, mitochondrial DNA and non-human DNA testing; Legal limits and issues in use of DNA forensics.

Peptide Synthesis | Thermo Fisher Scientific

In peptide synthesis diketopiperazine formation is a notorious side-reaction at the dipeptide stage and is particularly prone to occur in Fmoc based SPPS because of its mechanism.

Basic Peptides synthesis introduction - LifeTein®

The other approach is to introduce backbone protecting groups which will prevent the formation of hydrogen bonds. Such protection is made by the introduction of the Hmb group on the αnitrogen [53]. It has been shown that the presence of a Hmb unit every 6-7 residues is sufficient to disrupt the peptide aggregation [54]. The Hmb protected amino acid is introduced under the form of N,O-bis-Fmoc-N-(2hydroxy-4-methoxybenzyl) derivative, the O-Fmoc protection being cleaved during the following piperidine treatment. At the end of the synthesis the Hmb group is cleaved in the final TFA cleavage.

A Simple Introduction of Peptide Synthesis

This reaction occurs when Trp is used unprotected. The addition of Pmc (or Pbf) to the indole ring will occur and the amount of by-product is related to the distance between the Trp and the Arg residues [68].

helps to dictate the folding of the protein.

Historical background, Types, Manufacturing Methods, Functions, Typical Applications; Geosynthetic Testing, Physical, Mechanical, Construction survivability and durability testing of Geotextiles and Geogrids; Principles of Soil Reinforcement, Types of Reinforcement, Testing, Allowable loads for design (creep etc.), Elements of Design + NCMA Method, Fill Material, Detailed Design of Reinforced Soil Walls (BS 8006), Reinforced Slopes (BS 8006), Facia and Construction Methodology, Specifications, Bearing capacity Improvement, Embankments on soft soils, Basal Reinforcement – Design, Geocell – Design, Construction and Specification, IRC/MORTH Guidelines, Case Histories; Filteration and Drainage, Principles, Typical applications, Dams, French Drains, Testing, Design Principles, Specifications; Pavements and Airports; Influence of cyclic loading, Design for soft soils (unpaved) and new construction – Granular layers, GS in Bituminous Pavements – new construction and rehabilitation, Construction and specifications, Rural Road applications, Railway Tracks, Indian experiences; Natural fibre Materials, Jute and Coir Geotextiles - Products, Processes; Environmental Control, Erosion Control, Mechanics of Erosion, Methods of Control, Products, Design for various conditions, Silt Control, Land slides – Occurrence and methods of mitigation, Liners for Pounds and Canals; Engineered Landfills, Municipal Solid Waste, Hazardous Waste Landfills, Site location –MOEF Guidelines, Covers and liners, GCLs, Material aspects and, Design, construction and maintenance, the Indian Scenario

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