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PURExpress® In Vitro Protein Synthesis Kit | NEB

This became known as the “PURE” system, which stands for “Protein synthesis Using ..

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The PURE System for Protein Production | SpringerLink

PURExpress® is a reconstituted protein synthesis system based on the PUREsystem™ (Shimizu et al., 2001) where all necessary components needed for in vitro transcription and translation are purified from E. coli.

Mg 2+ and ATP/GTP concentrations on PURE system protein synthesis with Fluc as the reporter.

Advances in reconstituted CFPS systems such as the Protein synthesis Using Recombinant Elements (PURE) system offer new opportunities to tailor the reactions for specialized applications including in vitro protein evolution, protein microarrays, isotopic labeling, and incorporating unnatural amino acids.

The PURE System for Protein Production

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Recently, a cell-free protein synthesis system reconstituted solely from essential elements of the Escherichia coli translation system, termed protein synthesis using recombinant elements (PURE), has been widely used in synthetic biology to analyze fundamental life systems. Here, the system was integrated on a glass microchip system to construct a simple protein synthesis system. GFP template DNAs were immobilized on Sepharose microbeads by streptavidin–biotin binding. The beads were introduced into a Y-shaped microchannel in a glass microchip with a 10-μm height dam structure, and a PURE system reaction mixture was flowed through the microchannel. The recovered solutions had a higher fluorescent intensity than that of the reaction mixture before its introduction into the microchannel, thus verifying that GFP synthesis had been achieved. The microchip with DNA immobilized microbeads is reusable. This is advantageous over a conventional protein synthesis protocol requiring the preparation and addition of template DNA or mRNA into the reaction mixtures in aspect of simpleness and rapidness.

For structural genomics projects, the PURE system can be an alternative route to acquire difficult protein targets which resist traditional cellular expression (2).

Protein synthesis by pure translation systems - …

Protein synthesis by pure translation systems | Request …

PURExpress® from NEB is based on the PURESYSTEM® from PGI, and improves on the original “Classic II” Kit by optimizing components to increase yield of protein synthesis.

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cellular ribosomal protein synthesis system.
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  • Compared to the S30 system, protein synthesis in the ..

    12/02/2017 · Integration of a reconstituted cell-free protein-synthesis system on a glass microchip

  • Reconstitution of the protein synthesis system on a …

    13/01/2018 · Protein Synthesis in vitro: Cell-Free Systems Derived from Human Cells ..

  • Cell-free protein synthesis - Wikipedia

    was used as the DNA template for in vitro protein synthesis in the PURE system, ..

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The PURE Approach Return to Cell-Free ..

In this study, using firefly luciferase synthesis as a reporter system, we improved PURE system productivity up to 5 fold by adding or adjusting a variety of factors that affect transcription and translation, including Elongation factors (EF-Ts, EF-Tu, EF-G, and EF4), ribosome recycling factor (RRF), release factors (RF1, RF2, RF3), chaperones (GroEL/ES), BSA and tRNAs.

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