f Regulation of chitinase synthesis in Trichoderma harzianum
Regulation of 36-kDa β-1,3-glucanase synthesis in Trichoderma harzianum
of extracellular chitinase by Trichoderma harzianum TUBF 781 under ..
Production of extracellular endochitinase could be increased 5-fold in the mycoparasite fungus Trichoderma harzianum by using the cellulase promoter cbh1 of Trichoderma reesei, whereas the total endochitinase activity increased 10-fold. The cbh1 promoter was not expressed on glucose and sucrose in T. harzianum and was induced by sophorose and on cellulase-inducing medium. The endogenous endochitinase gene was expressed at a low basal level on glucose and sucrose. No specific induction by crab shell chitin or sophorose was observed.
A wide range of effect of pH on chitinase activity was shown by SR1 (Table ). The rate of hydrolysis of chitin was increased almost linearly between pH 3.5–5.0 in both still and shake cultures. A fairly broad optimum pH occurred with maximal activity at pH 5.0. Beyond which the activity slowly declined. Initial pH value of the culture medium of pH 5.0 showed the highest chitinolytic activity in 7-day-old shaken culture using Aspergillus carneus strain . Studies over a different periods of incubation of SR1, determined the optimum temperature of chitinase activity was at 30°C (Table ). The optimum temperature reflects a balance between the effect on the activity and the maximum yield of the enzyme protein. This was positively correlated on the chitinase production by Trichoderma harzianum in submerged fermentation [, ].
of chitinase synthesis in Trichoderma harzianum, J Gen ..
Moreover, botanical aromatics, furfural, citral and benzaldehyde showed potential for control of both fungal pathogens and phytoparasitic nematodes and they did not reduce colonization of cotton roots by plant growth promoting rhizobacteria (PGPR).
These antagonists were isolated fromcucumber, cantaloupe, tomato and pepper grown in plastic houses under protected cultivation systems and showing root rot disease symptoms.
Regulation of chitinase synthesis …
Cucumber, Cantaloupe, Tomato and Pepper grown in plastic houses under protected cultivation system and showing root rot and or damping-off disease symptoms.The isolated fungi proved their aggressive ability to induce root rot disease of those vegetables.
Regulation of chitinase synthesis in Trichoderma harzianum.
PRODUCTION OF CHITINOLYTIC ENZYMES BY TRICHODERMA HARZIANUM
26/06/2000 · Regulation of chitinase 33 (chit33) gene expression in Trichoderma harzianum
Regulation of chitinase synthesis in ..
01/02/2014 · Isolation and characterization of three chitinase in Trichoderma harzianum ..
chitinase synthesis in Trichoderma harzianum.
Isolation and characterization of three chitinase in Trichoderma harzianum
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