This membrane bound organelle is the site of lipid synthesis
Smooth Endoplasmic Reticulum (sER) Lipid synthesis takes place at the interface of the sER membrane and the cytosol
Synthesis of Lipids | Biosynthesis | Lipid - Scribd
purified a phospholipid (phosphatidylcholine) from egg yolk onan alumina column ().
Baer E described the synthesis of phosphatidic acid by treatment ofdiacylglycerol with diphenylchlorophosphate and removal of the phenyl group bycatalytic reduction ().
Hofman K et al.
described for the first time the synthesis of a naturalneutral lipid, tributyrin, by the direct esterification of glycerol and butyricacid ().
12/01/2018 · Synthesis of Lipids: Biochemistry ..
Biological membranes are composed of lipids and proteins that together form hydrophobic barriers that limit the distribution of aqueous macromolecules and metabolites. Cells use membranes for a number of different purposes, including segregation and protection from the environment, compartmentalization of functions, energy production, storage, protein synthesis and secretion, phagocytosis, movement, and cell-cell interaction. Eukaryotic cells contain ordered infrastructures, called organelles, to organize and carry out complex processes and to enable distinct reactions that require a hydrophobic environment. The level and complexity of compartmentalization varies among organisms and among mammalian cells. Some cells also change in size and organelle complexity after biological stimulation. An example of induced membrane biogenesis occurs in naïve B-lymphocytes that are converted to plasma cells (), and an example of membrane redistribution occurs in macrophages in which the Golgi apparatus is reoriented during transient cytokine synthesis and secretion (). The versatility of biological membranes is dependent on their structures and biophysical properties, which are dictated by the types of lipids and proteins that compose the membranes. The functions of membranes require a fluid plasticity that is accomplished through alteration in lipid composition. Lipid composition is diverse, not only among different organisms, but also among different compartments within the same cells and between the two leaflets of the same membrane. Lipid composition is determined through regulation of de novo synthesis at designated cellular sites, selective distribution or trafficking to new sites, and by localized remodeling reactions. Understanding the relationships between the dynamic changes in membrane lipid composition and specific cellular events is our current challenge. This review is focused on membrane phospholipid biogenesis in mammalian cells with a particular emphasis on the role played by the endoplasmic reticulum (ER). The ER, together with the Golgi apparatus, is a major site of de novo bulk membrane lipid synthesis, and recent experiments demonstrate a link between phospholipid synthesis and secretion from this compartment.
The ER and Golgi apparatus together constitute the endomembrane compartment in the cytoplasm of eukaryotic cells. The endomembrane compartment is a major site of lipid synthesis, and the ER is where not only lipids are synthesized, but membrane-bound proteins and secretory proteins are also made. The ER is organized into a labyrinthine membrane-bound network of branching tubules and flattened sacs that extends throughout the cytosol. The tubules and sacs interconnect, and their membrane is continuous with the outer nuclear membrane (). ER and nuclear membranes form a continuous sheet enclosing a single internal space, called the lumen. The ER can be divided into subdomains in relation to their function or location. The nuclear envelope is the domain that separates the genetic material from the cytosol. The ribosomes that synthesize ER-associated proteins are attached to the cytoplasmic aspect of the ER membrane, and these regions are designated as rough ER. The transitional ER is characterized by two domains, namely, a domain associated with ribosomes at a low density and a region that lacks attached ribosomes, called smooth ER. The ER region in close proximity with the mitochondrium is the mitochondrium-associated membrane. Finally, the region in close proximity to the Golgi apparatus, rich in vesicles and tubules, is the ER-Golgi intermediate compartment (ERGIC) (). The ERGIC domain represents a continuum of the ER and Golgi apparatus where the lipids and lumenal proteins destined for transport to the cell surface or other organelles are transferred and biochemically modified. The cis-Golgi structure is in close proximity to the ERGIC, and the trans-Golgi network is the site for the formation of budding vesicles that distribute the lumenal protein contents. The ER interacts closely with the cytoskeleton, mostly with microtubules. This interaction allows the ER to maintain its position within the cell and facilitates intracellular trafficking, particularly from the smooth ER ().
Glossary | Linus Pauling Institute | Oregon State University
GPAT and AGPAT activities begin the process of glycerolipid synthesis by attaching the fatty acyl moieties to the 1-position and then the 2-position of glycerol-3-phosphate, respectively. GPAT and AGPAT activities are associated with the ER and the mitochondria, providing the diacylglycerol phosphate (DGP) precursor for phospholipids in both locations. In the ER compartment, the DGP is dephosphorylated by the phosphatidic acid phosphatase enzymes to yield diacylglycerol (DG), which is incorporated into phosphatidylcholine (DGPCho) and phosphatidylethanolamine (DGPEtn). The GPAT and AGPAT association with the mitochondria suggests that these activities provide the DGP precursor for the synthesis of phosphatidylglycerol (DGPGro) and CL located at the same site.
DGPEtn is the second most abundant glycerophospholipid species, and its de novo synthesis can be catalyzed by the CEPT, located in the ER, or the EPT, a recently described isoform with strict specificity for DGPEtn production (). An EPT activity has been described that is associated with peroxisomes (). DGPEtn can also arise from head-group exchange with phosphatidylserine (DGPSer) in the ER as mediated by PSS2 or in the mitochondria by DGPSer decarboxylation, mediated by PSD (). Differently from DGPCho and DGPEtn, DGPSer is synthesized in the ER through head-group exchange of already-made DGPCho and DGPEtn as catalyzed by PSS1 and PSS2, respectively (). Triacylglycerol has no structural role but serves primarily as a storage lipid and is also synthesized in the ER (). Phosphatidylinositol (DGPIns) is synthesized in the ER by the PIS, and, apart from phosphatidylinositol-4-phosphate, its conversion into the highly phosphorylated forms, which play critical roles in signaling and membrane vesicle trafficking, occurs outside the ER (, , , ). CL is present only in the mitochondria, where it is absolutely required for energy production, and its synthesis is restricted to the inner mitochondrial membrane (, , ). Plasmalogens, which are vinyl-ether linked at the 1-position of the glycerophospholipid, are an important class of lipids, and they contribute almost 18% to the total lipid mass in humans. Among plasmalogens, plasmenylethanolamine (PlmePEtn) is the most abundant and it is also the precursor to plasmenylcholine (PlmePCho). Plasmalogen synthesis occurs in the peroxisomes, where the key enzyme GNPAT initiates formation using a fatty alcohol to yield AlkylGnP ().
Journal of Lipid Research :: About the JLR
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Sphingolipid synthesis spans from the ER, where it begins, to the Golgi complex, where it ends (, ). Synthesis of the sphingosine and ceramide (Cer) intermediates occurs in the ER. Cer is then transferred to the Golgi apparatus in two manners, and each mode determines whether Cer is converted into either sphingomyelin (CerPCho) or glucosylceramide (GlcCer) and lactosylceramide (LacCer). Lipids such as DGPCho and DGPIns can be synthesized in the nuclear matrix apart from the nuclear envelope, but information about the exact location of the nuclear enzymes is limited ().
29/04/2010 · Original Article
Phospholipids, including glycerophospholipids and sphingolipids, constitute the bulk lipid components of all mammalian membranes. Based on the information contained in several previous reviews (–), the phospholipid biosynthetic enzymes that produce membrane lipid products have been assigned to the different organelles in according to where the majority of protein or activity for each has been measured. Enzymes involved in phospholipid degradation or remodeling are not addressed. Enzymes that synthesize unique glycerophospholipids, called plasmalogens, are included. Water soluble intermediates are not shown in the scheme, with the exception of fatty acyl-CoA, which is used as substrate by acyltransferase enzymes for the synthesis of both glycerolipids and sphingolipids in the ER, cardiolipin (CL) and phosphatidylglycerol (DGPGro) in mitochondria, and for the synthesis of plasmalogens (PlmePEtn and PlmePCho) in the peroxisomes. The family of acyltransferases is quite extended, and only a few isoforms are directly involved in the de novo synthesis of membrane lipids, while others are involved in the remodeling of acyl chains of the different lipid classes (, ). The acronyms for the different lipids are those proposed by the Lipid Maps project (), and the corresponding nomenclature for the lipid biosynthetic enzymes are defined in . Different isoforms of the lipid biosynthetic enzymes are not pointed out unless they are known to have alternate substrate specificities. Rather, the goal is to obtain a holistic view of the overall process of membrane lipid biogenesis.
Effects of Combination Lipid Therapy in Type 2 Diabetes Mellitus
Although different lipids are synthesized in different organelles, they are widely distributed within the cell and the membrane composition of the different organelles does not necessarily reflect their lipid biosynthetic capacity. DGPCho is synthesized in the endomembrane compartment and in the nuclear compartment in immortalized cells, but it is present everywhere in the cell. DGPSer and CerPCho are synthesized in the ER and Golgi, but they are highly abundant in the plasma membrane. PlmePEtn is synthesized in the peroxisomes but does not accumulate as it is primarily secreted. Within the same membrane, lipids are transported to or segregated into one of the two leaflets of the membrane by virtue of their chemical structure or by the action of enzymes called flippases, whose function is to favor or force the movement of specific lipids between the two leaflets of the membrane (, ). The transport of lipids to different membranes can occur through the vesicular pathways, which allow the transport of membrane to even distant cellular locations or by lipid-transfer proteins, a process that is particularly active and fast within membrane contact sites (MCS), where membrane regions from different organelles come in close proximity (within 10 nm) to one another (). For example, the ER is known to generate MCS structures with mitochondria, plasma membrane, the Golgi apparatus, endosomes, and other organelles. The means by which DGPCho and DGPEtn are transported to the peroxisomes is still unknown, and MCS structures as well as vesicles may be responsible for mediating the process.
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