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KW - Fluorescence-activated cell sorting

Reagent for fluorescent labelling of proteins: Anal. Biochem., 20, 178 (1968).

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Fluorescein isothiocyanates: Improved synthesis and …

The tetrachloro (TET)-, hexachloro (HEX) - and dichloro-dimethoxy (JOE)- fluorescein phosphoramidites are designed to take advantage of the multicolor detection capability of modern DNA sequencers and genetic analyzers.

Potential nutriceuticalAllyl isothiocyanate is a volatile organic compound.

Jeong and coworkers have developed oleyl-chitosan (oleic acid-conjugated chitosan) SPIONs []. In aqueous solution, oleyl-chitosan could self-assemble into core-shell structures and provide the core compartment for the loading of SPIONs. Oleyl-chitosan@ SPION showed good enhanced MRI sensitivity in MRI. Cy 5.5 dye was functionalized on the oleyl-chitosan for near-infrared (NIR) optical imaging. SPION-loaded Cy5.5-conjugated oleyl-chitosan NPs were injected intravenously into a tumor-bearing mice. Both NIR fluorescence and MR imaging showed the detectable signal intensity and enhancement in tumor tissues via enhanced permeability and retention (EPR) effect (Figure ).

Fluorescein isothiocyanates: Improved synthesis and ..

Our fluorescein phosphoramidite also contains a DMT group to allow quantification of coupling.

The fluorescein alternative, Gig Harbor Green, has been discontinued.

The Eclipse quencher from ELITechGroup solves most of the problems inherent in the synthesis of molecular beacon and FRET probes.

We are pleased to offer products based on ELITechGroup's Redmond Red®, Yakima Yellow® and AquaPhluor® 593 fluorophores and Eclipse® non-fluorescent quencher.

of a hydrochloride of fluorescein isothiocyanate.

Fluorescein CPG has traditionally been used to add the fluorescein label at the 3'-terminus.

(A) HRTEM image and (B) EDP analysis of Fe3O4-CdSe heterodimers. (C) The UV-Vis and fluorescence spectra of Fe3O4-CdSe NPs in hexane solution. (D) The fluorescence images of the hexane solution of Fe3O4-CdSe NPs before and after attraction by a small magnet (excited by a UV lamp, λex = 365 nm). Reproduced with permission from Ref. []. Copyright (2008) American Chemical Society.

PLMA, biodegradable polyester, is biocompatible and easy to be bifunctionalized through conjugating other imaging probes. Wang and coworkers [] coated magnetic NPs with PLMA covalently bound fluorescein isothiocyanate (FITC) (FITC-PLMA-MNPs) by ligand exchange method (Figure ). The FITC-PLMA-MNPs labeled hMSCs could be observed under confocal fluorescence microscopy and imaged by a 1.5 T MR scanner at a threshold cell number of 1200 without adverse effects on the osteogenic and adipogenic differentiation potentials of hMSCs.

Fluorescein-dT CPG allows both of these enzymatic activities to proceed.
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  • Allyl isothiocyanate (stabilised) for synthesis

    We also offer 3'-(6-FAM) CPG and Fluorescein-dT CPG, both derivatives of 6-carboxyfluorescein (6-FAM).

  • Phalloidin, Fluorescein Isothiocyanate Labeled.

    The fluorescent dyes, Yakima Yellow, Redmond Red and AquaPhluor 593, are available as phosphoramidites and supports.

  • 6-FITC Fluorescein Dye Oligonucleotide Labeling

    This may reflect the BHQ's ability to form stable, non-fluorescent complexes which can be a plus even in FRET probes.

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LifeTein Fluorescent Peptide ..

Zhang reported PEG coated SPIONs for both MRI and optical imaging. The biocompatible PEG coating bearing amine functional group could serve as a platform to incorporate a variety of targeting, therapeutic or imaging ligands []. In this case, chlorotoxin was conjugated to PEG@SPION and Cy5.5 (a near-infrared fluorescent dye). These SPIONs have shown specifically to accumulate in xenograft tumors of a brain tumor mouse model. Furthermore, they did not have any toxicity or negative health effects from the results of histopathology and blood toxicity assays. Huang and coworkers have designed hyaluronic acid (HA) coated SPIONs for targeting activated macrophages []. The HA-coated SPIONs had specific biological recognition with the receptor CD44. The cell uptake studies showed a significant uptake of SPIONs by activated macrophage cell line THP-1 and enabled MRI of THP-1 cells. The dual modal probes could be used to track the magnetite core and cargo individually. The magnetite core was only present inside the cells while the cargo fluorescein was found to be delivered to the cell nucleus. This study reveals the fact that the HA-based SPIONs have great potential in nucleus targeting drug delivery.

for example fluorescein isothiocyanate, ..

Howes reported a simple synthesis of multicolored bright fluorescent-magnetic NPs by encapsulating hydrophobic conjugated polymers and SPIONs in phospholipid micelles []. These SPIONs were shown to be magnetically responsive and simultaneously fluorescent as bimodal imaging agents. These bifunctional NPs had shorter effect on the transverse 2* relaxation time and yet showed their potential as MRI contrast agent. They were also successfully taken up by SH-SY5Y neuroblastoma cells and showed their potential in fluorescence imaging.

Synthesis and characterization of fluorescein ..

Our group has been actively involved in the synthesis of QDs and magnetic QDs (MQDs) [-]. We have successfully demonstrated the magnetic and fluorescent properties of Fe2O3-CdSe MQDs, silica -coated QDs or MQDs and their application in cell labeling (Figure ) []. The silanization using aminopropyl triethoxysilane (APS) in a reverse microemulsion produced thin silica coating on bare CdSe QDs or Fe2O3-CdSe MQD with surface NH2 groups. The methoxy groups of APS were hydrolyzed and condensed with another APS, exposing surface amine groups on the silanized QDs (SiO2/QDs) for conjugation with oleyl-O-poly(ethyleneglycol)-succinyl-N-hydroxysuccinimidyl ester, denoted as bio-anchored membrane (BAM). The reaction between the amine group and NHS ester resulted in a covalent amide bond formation, leaving the exposed oleyl group for the effective targeting of cell membrane. The labeling of live cell membranes (HepG2 human liver cancer cells and NIH-3T3 mouse fibroblast cells) using confocal laser scanning microscopy (CLSM) indicated the successful conjugation of silica-coated QDs or MQDs with BAM.

Difference between fluorescein and FITC labeling

The fluorescent (CdSe QDs) and magnetic (Fe3O4 SPIONs) heterodimers were fabricated in the group of Xu and were used for the intracellular tracking. Figure details the HRTEM, selected area electron diffraction (SAED), absorption and fluorescence spectra of Fe3O4-CdSe NPs in hexane solution, and the optical photographs illustrating the fluorescence and magnetic properties [].

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